PT Journal
AU Ogburia, M
TI Somatic Embryogenesis, Plantlet Regeneration and Micropropagation of Cultivars and F<sub>1</sub> Hybrids of Manihot esculenta
SO Biologia plantarum
PY 2003
BP 429
EP 432
VL 46
IS 3
DI 10.1023/B:BIOP.0000023888.82993.b1
WP https://bp.ueb.cas.cz/artkey/bpl-200303-0042.php
DE 2; 4-dichlorophenoxyacetic acid; in vitro growth; 1-naphthaleneacetic acid; organogenesis; phytohormones
AB Explants of four F<sub>1</sub> hybrids (OMR 36-41/1, OMR 36-41/2, OMR 36-41/4 and OMR 36-41/5) and two cultivars (Rayong 1 and Rayong 60) of cassava (Manihot esculenta Crantz) were subjected to different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), kinetin (KIN) and N<sup>6</sup>-benzylaminopurine (BAP) to induce somatic embryogenesis, organogenesis and micropropagation. Shoot apices of the F<sub>1</sub> hybrids exhibited higher frequency (62 - 74 %) of proliferation of somatic embryos than the cultivars (21 - 43 %) in Murashige and Skoog basal medium supplemented with 8 mg dm<sup>-3</sup> 2,4-D and 0.5 mg dm<sup>-3</sup> NAA. Nodal explants of regenerated plantlets were rapidly micropropagated with 90 % efficiency on a medium containing 0.1 mg dm<sup>-3</sup> NAA and 0.05 mg dm<sup>-3</sup> BAP irrespective of explant source.
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