RT Journal Article
SR Electronic
A1 Ogburia, M.N.
T1 Somatic Embryogenesis, Plantlet Regeneration and Micropropagation of Cultivars and F&lt;sub&gt;1&lt;/sub&gt; Hybrids of Manihot esculenta
JF Biologia plantarum
YR 2003
VO 46
IS 3
SP 429
OP 432
DO 10.1023/B:BIOP.0000023888.82993.b1
UL https://bp.ueb.cas.cz/artkey/bpl-200303-0042.php
AB Explants of four F&lt;sub&gt;1&lt;/sub&gt; hybrids (OMR 36-41/1, OMR 36-41/2, OMR 36-41/4 and OMR 36-41/5) and two cultivars (Rayong 1 and Rayong 60) of cassava (Manihot esculenta Crantz) were subjected to different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), kinetin (KIN) and N&lt;sup&gt;6&lt;/sup&gt;-benzylaminopurine (BAP) to induce somatic embryogenesis, organogenesis and micropropagation. Shoot apices of the F&lt;sub&gt;1&lt;/sub&gt; hybrids exhibited higher frequency (62 - 74 %) of proliferation of somatic embryos than the cultivars (21 - 43 %) in Murashige and Skoog basal medium supplemented with 8 mg dm&lt;sup&gt;-3&lt;/sup&gt; 2,4-D and 0.5 mg dm&lt;sup&gt;-3&lt;/sup&gt; NAA. Nodal explants of regenerated plantlets were rapidly micropropagated with 90 % efficiency on a medium containing 0.1 mg dm&lt;sup&gt;-3&lt;/sup&gt; NAA and 0.05 mg dm&lt;sup&gt;-3&lt;/sup&gt; BAP irrespective of explant source.