bpl-200303-0042 ArticleInAPeriodical 2003 Biologia plantarum 46 3 https://bp.ueb.cas.cz/artkey/bpl-200303-0042.php https://doi.org/10.1023/B:BIOP.0000023888.82993.b1 429-432 OgburiaM.N. Somatic Embryogenesis, Plantlet Regeneration and Micropropagation of Cultivars and F₁ Hybrids of Manihot esculenta Explants of four F₁ hybrids (OMR 36-41/1, OMR 36-41/2, OMR 36-41/4 and OMR 36-41/5) and two cultivars (Rayong 1 and Rayong 60) of cassava (Manihot esculenta Crantz) were subjected to different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), kinetin (KIN) and N⁶-benzylaminopurine (BAP) to induce somatic embryogenesis, organogenesis and micropropagation. Shoot apices of the F₁ hybrids exhibited higher frequency (62 - 74 %) of proliferation of somatic embryos than the cultivars (21 - 43 %) in Murashige and Skoog basal medium supplemented with 8 mg dm^-3 2,4-D and 0.5 mg dm^-3 NAA. Nodal explants of regenerated plantlets were rapidly micropropagated with 90 % efficiency on a medium containing 0.1 mg dm^-3 NAA and 0.05 mg dm^-3 BAP irrespective of explant source.