PT Journal
AU Prabu, G
   Thirugnanasambantham, K
   Mandal, A
   Saravanan, A
TI Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea
SO Biologia plantarum
PY 2012
BP 140
EP 144
VL 56
IS 1
DI 10.1007/s10535-012-0030-5
WP https://bp.ueb.cas.cz/artkey/bpl-201201-0023.php
DE Camellia sinensis; cytosolic expression; nucleoside triphosphate pool; sequence analysis; Southern blot
AB Nucleoside diphosphate kinase (NDPK) operates in the homeostasis of cellular nucleoside triphosphate (NTP) pools and the cytosolic NDPK1 is the main NDPK isoform in plants, accounting for more than 70 % of total NDPK activity in plant. A full length cDNA (697 bp), designated as CsNDPK1 was cloned from Camellia sinensis (L.) O. Kuntze leaves. Sequence analysis of CsNDPK1 shows several motifs, binding and catalytic sites which are highly conserved among other NDPKs. Southern blot analysis revealed that tea genome has two copies of CsNDPK1. Transcription pattern analysis indicated that CsNDPK1 is expressed in all tissues examined, but expressed more in buds than in other organs.
ER