RT Journal Article
SR Electronic
A1 Prabu, G. R.
A1 Thirugnanasambantham, K.
A1 Mandal, A. K. A.
A1 Saravanan, A.
T1 Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea
JF Biologia plantarum
YR 2012
VO 56
IS 1
SP 140
OP 144
DO 10.1007/s10535-012-0030-5
UL https://bp.ueb.cas.cz/artkey/bpl-201201-0023.php
AB Nucleoside diphosphate kinase (NDPK) operates in the homeostasis of cellular nucleoside triphosphate (NTP) pools and the cytosolic NDPK1 is the main NDPK isoform in plants, accounting for more than 70 % of total NDPK activity in plant. A full length cDNA (697 bp), designated as CsNDPK1 was cloned from Camellia sinensis (L.) O. Kuntze leaves. Sequence analysis of CsNDPK1 shows several motifs, binding and catalytic sites which are highly conserved among other NDPKs. Southern blot analysis revealed that tea genome has two copies of CsNDPK1. Transcription pattern analysis indicated that CsNDPK1 is expressed in all tissues examined, but expressed more in buds than in other organs.