PT  - JOURNAL ARTICLE
AU  - Mimida, N.
AU  - Li, J.
AU  - Zhang, C.
AU  - Moriya, S.
AU  - Moriya-Tanaka, Y.
AU  - Iwanami, H.
AU  - Honda, C.
AU  - Oshino, H.
AU  - Takagishi, K.
AU  - Suzuki, A.
AU  - Komori, S.
AU  - Wada, M.
TI  - Divergence of TERMINAL FLOWER1-like genes in Rosaceae
DP  - 2012 Sep 1
TA  - Biologia plantarum
PG  - 465--472
VI  - 56
IP  - 3
AID - 10.1007/s10535-012-0113-3
IS  - 
AB  - Rosaceae is a large family, however, our understanding of its phylogeny is based largely on morphological observations. To understand the relationship between subfamilies Rosoideae, Amygdaloideae, Maloideae and Spiraeoideae at a molecular level, we isolated and compared the plant phosphatidyl ethanolamine-binding protein-like genes TERMINAL FLOWER1 (TFL1)-like and CENTRORADIALIS (CEN)-like, which are involved in the control of shoot meristem identity and flowering time. A comparison of gene structures and phylogenetic tree analyses by the Neighbor-Joining method showed that each of the two TFL1-like (MdTFL1-1 and MdTFL1-2) and CEN-like genes (MdCENa and MdCENb) in Maloideae were classified into two distinct clades. The TFL1-like and CEN-like genes of Gillenia in Spiraeoideae belonged to monophyletic Maloideae groups, suggesting that Gillenia and Maloideae have a common near ancestor. However, the Gillenia TFL1-like gene does not contain the insertion sequence of the third intron that is found in MdTFL1-2-like genes of the members of Maloideae such as apple, Korean whitebeam, quince, and Siberian mountain ash. Therefore, after the Maloideae ancestor genome became polyploid through hybridization between Gillenia-like species or genome doubling, an insertion sequence of the third intron of MdTFL1-2-like genes was generated.