RT Journal Article
SR Electronic
A1 Yang, D.-Y.
A1 Ma, N.-N.
A1 Liu, Z.-M.
A1 Ma, X.-C.
A1 Zhao, S.-J.
A1 Meng, Q.-W.
T1 Suppression of tomato SlGGP aggravates methyl viologen-mediated oxidative stress
JF Biologia plantarum
YR 2016
VO 60
IS 4
SP 677
OP 685
DO 10.1007/s10535-016-0628-0
UL https://bp.ueb.cas.cz/artkey/bpl-201604-0008.php
AB Ascorbate (AsA) is an important antioxidant that can scavenge reactive oxygen species to protect plant cells against oxidative stress. Guanosine 5'-diphosphate (GDP)-L-galactose phosphorylase (GGP) is a key enzyme in the AsA biosynthetic pathway. To investigate the functions of GGP in AsA synthesis and oxidative stress tolerance in tomato, antisense lines with a reduced expression of SlGGP were obtained. Photobleaching after treatment of leaf disks with methyl viologen was more severe in transgenic lines compared to wild type (WT) plants. Moreover, compared with the WT plants, the transgenic plants showed a higher content of hydrogen peroxide, superoxide anion, malondialdehyde, as well as ion leakage, but a lower content of AsA and chlorophylls, ascorbate peroxidase activity, net photosynthetic rate, and maximal photochemical efficiency of photosystem II. Results of real-time quantitative polymerase chain reaction show that suppression of the SlGGP gene in the transgenic plants reduced their oxidative stress tolerance.