biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 35:151-155, 1993 | DOI: 10.1007/BF02921141

Quantitative β-glucuronidase assay in transgenic plants

S. Vitha1, K. Beneš1, M. Michalová1, M. Ondřej1
1 Institute of Plant Molecular Biology, Academy of Science of the Czech Republic, České Budějovice, Czech Republic

Several factors influencing reliability of the quantitative fluorimetric β-glucuronidase (GUS) assay in transgenic plant tissue have been investigated. We obtained linear dependence of fluorescence on both the duration of hydrolysis and the extract concentration. The stability of the enzyme in the homogenate was fairly high, the same as the stability of the substrate solution and of the final reaction product. The modification of the extraction/incubation buffer was proposed, resulting in several times higher activity in comparison with original procedure.

Received: November 28, 1991; Accepted: February 14, 1992; Published: March 1, 1993  Show citation

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Vitha, S., Beneš, K., Michalová, M., & Ondřej, M. (1993). Quantitative β-glucuronidase assay in transgenic plants. Biologia plantarum, 35(1), 151-155. doi: 10.1007/BF02921141
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    References

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