Biologia plantarum, 2026 (vol. 70)
Original Papers
Optimized protocol for in vitro indirect organogenesis and shoot regeneration of Platycladus orientalis
Luis LUCENA-SORIANO, Ana María FERNÁNDEZ-OCAÑA
Biologia plantarum 70:1-10, 2026 | DOI: 10.32615/bp.2026.001 
Background: Platycladus orientalis L. is a drought-tolerant conifer valued for its ornamental and medicinal properties. However, efficient regeneration systems for this species remain limited, hindering its propagation and conservation. Aims: The aim of this study was to develop a reliable protocol for indirect organogenesis of Platycladus orientalis under in vitro conditions, evaluating the influence of explant type, culture medium, light exposure, and pretreatment on regeneration efficiency. Methods: Cotyledon, hypocotyl, and radicle explants were cultured on different media formulations. The effects of light and darkness during...
RT-qPCR analysis of genotype-specific beet yellows virus accumulation in sugar beet
Emad IBRAHIM, Dilara BOZDOĞAN, Jiban KUMAR KUNDU
Biologia plantarum 70:11-17, 2026 | DOI: 10.32615/bp.2026.002
Background: Rapid and objective characterisation of viral accumulation requires methods combining fast detection and temporal resolution. Aims: Develop a two-phase approach for screening sugar beet genotypes for beet yellows virus (BYV) accumulation dynamics. Methods: Phase 1 employed one-enzyme RTX-PCR for rapid BYV detection, followed by single-time-point RT-qPCR at 30 days post-inoculation (dpi) to screen ten genotypes. Phase 2 performed time-course RT-qPCR at six time points (10-60 dpi) on two contrasting genotypes selected from Phase 1, with assay performance validated by a standard curve. Results: One-enzyme RTX-PCR confirmed BYV infection, enabling...