Biologia plantarum 36:359-364, 1994 | DOI: 10.1007/BF02920932
Aspartate aminotransferase isozymes in plants: Comparison of two staining methods in polyacrylamide gels
- 1 OSEVA, Research Institute of Technical Crops and Legumes, Sumperk, Czech Republic
Two staining methods for aspartate aminotransferase were compared after electrophoretic resolution of its isozymes in polyacrylamide gels. The first one uses L-aspartic acid and Fast Blue BB salt (classical method), the second uses L-cysteine sulfinic acid and a redox system with phenazine methosulfate and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide. The seeds of pea, horse bean and soybean were used as a model plant source of the enzyme. The staining method with L-cysteine sulfinic acid is very reliable and more sensitive than the Fast Blue BB method and allows detection at very low isozyme activities in the gel.
Received: September 24, 1993; Accepted: October 25, 1993; Published: September 1, 1994 Show citation
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