biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:481-489, 1995 | DOI: 10.1007/BF02908823

PCR derived molecular markers and phylogenetic relationships in theSecale genus

J. C. Del Pozo1, A. M. Figueiras1, C. Benito1, A. De La Peña1
1 Departamento de Genética, Facultad de Biologia, Universidad Complutense Madrid, Madrid, Spain

DNA from 22 different species, accessions, cultivars and lines included in theSecale genus were analyzed by the polymerase chain reaction (PCR), using as primers five pairs of oligonucleotides derived from specific sequences. A total of 42 amplified bands were considered, and some of them appeared to be potentially useful as molecular markers for some of the analyzed groups. These amplified bands were used to generate molecular phenograms inside theSecale genus.

Keywords: genetic markers; PCR band polymorphism; rye genetic diversity; rye phenograms

Received: February 8, 1995; Accepted: May 29, 1995; Published: December 1, 1995  Show citation

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Del Pozo, J.C., Figueiras, A.M., Benito, C., & De La Peña, A. (1995). PCR derived molecular markers and phylogenetic relationships in theSecale genus. Biologia plantarum, 37(4), 481-489. doi: 10.1007/BF02908823
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