biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 39:575-582, 1997 | DOI: 10.1023/A:1000978715531

Subcellular localization and polymorphism of peroxidase in horse-radish tumour and teratoma tissue

T. Pe¹kan1, M.A. Pedreño1, M. Krsnik-Rasol1, R. Muñoz1
1 Departamento de Biología Vegetal, Facultad de Biologia, Universidad de Murcia, Espinardo, Murcia, Spain

The localization of peroxidase in cells of horse-radish (Armoracia lapathifolia Gilib.) tumour and teratoma tissues was studied. Both tissue lines were derived from the same primary crown-gall tumour induced on the leaf fragments by a wild type of Agrobacterium tumefaciens B6S3. Enzymatic activity was measured in cell walls, high-density heterogeneous membrane fraction, microsomal and soluble (no particulate) fractions. The subcellular localization of enzymatic activity was distinct for each transformed tissue. Both tumour and teratoma showed similar isoenzyme patterns, but one soluble acidic isoperoxidase could be considered as a marker of cell differentiation.

Keywords: Agrobacterium tumefaciens; Armoracia lapathifolia; cell differentiation; tumour growth
Subjects: Agrobacterium tumefaciens; Armoracia lapathifolia; cell differentiation marker, acidic isoperoxidase; horse-radish tumour, peroxidase; teratoma, peroxidase localization; tumour growth, peroxidase; tumour, peroxidase localization

Prepublished online: May 1, 1997; Published: December 1, 1997  Show citation

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Pe¹kan, T., Pedreño, M.A., Krsnik-Rasol, M., & Muñoz, R. (1997). Subcellular localization and polymorphism of peroxidase in horse-radish tumour and teratoma tissue. Biologia plantarum, 39(4), 575-582. doi: 10.1023/A:1000978715531
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    References

    1. Biggs, K.J., Fry S.C.: Phenolic cross-linking in the cell wall.-In: Cosgrove, D.J, Knievel, D.P. (ed.): Physiology of Cell Expansion During Plant Growth. Pp. 46-57. American Society of Plant Physiologists, Rockville 1987.
    2. Cooper, B., Varner, J.E.: Cross-linking of soluble extensin in isolated cell walls.-Plant Physiol. 76: 414-417, 1984. Go to original source...
    3. Everdeen, D.S.I., Kiefer, S., Willard, J.J., Muldoon, E.P., Dey, P.M., Li, X.B., Lamport, D.T.A.: Enzyme cross-linkage of monomeric extensin precursors.-Plant Physiol. 87: 616-621, 1988. Go to original source...
    4. Ferrer, M.A., Pedreño, M.A., Muñoz, R., Ros Barceló, A.: Oxidation of coniferyl alcohol by cell wall peroxidases at the expense of the indole-3-acetic acid and O2. A model for the lignification of cell walls in the absence of H2O2.-FEBS Lett. 276: 127-130, 1990. Go to original source...
    5. Ferrer, M.A., Pedreño, M.A., Muñoz, R., Ros Barceló, A.: Soluble peroxidase gradients in lupin hypocotyl and the control of polarly transported indole3-yl acetic acid.-J. Plant Growth Regul. 10: 139-146, 1991a. Go to original source...
    6. Ferrer, M.A., Calderón, A.A., Muñoz, R., Ros Barceló, A.: 4-methoxy-α-naftol as a specific substrate for kinetic, zymographic and cytochemical studies on plant peroxidase activities.-Phytochem. Anal. 1: 63-69, 1991b. Go to original source...
    7. Ferrer, M.A., Pedreño, M.A., Ros Barceló A., Muñoz, R.: The cell wall localization of two strongly basic isoperoxidases in etiolated hypocotyls and its significance in coniferyl alcohol oxidation and indole-3-acetic acid catabolism.-J. Plant Physiol. 139: 611-616, 1992. Go to original source...
    8. Fransz, P.F., De Ruijter, N.C.A., Schel, J.H.N.: Isozymes as biochemical and cytochemical markers in embryogenic callus culture of maize (Zea mays L.).-Plant Cell Rep. 8: 67-70, 1989. Go to original source...
    9. Fry, S.C.: Roles of the primary cell walls in morphogenesis.-In: Nijkamp, H.J.J, Van der Plas, L.H.W., Van Aartrick, J. (ed.): Progress in Plant, Cellular and Molecular Biology. Pp. 504-513. Kluwer Academic Publishers, Dordrecht 1990. Go to original source...
    10. García-Florenciano, E., Calderón, A.A., Pedreño, M.A., Muñoz, R., Ros Barceló, A.: The vacuolar localization of basic isoperoxidases in grapevine cell cultures and its significance in indole-3-acetic acid catabolism.-Plant Growth Regul. 10: 125-138, 1991. Go to original source...
    11. García-Florenciano, E., Calderón, A.A., Muñoz, R., Ros Barceló, A.: The decarboxylative pathway of indole-3-acetic acid is not functional in grapevine protoplasts.-J. exp. Bot. 43: 715-721, 1992. Go to original source...
    12. Gaspar, T., Penel, C., Greppin, H.: Peroxidases 1970-80. A Survey of Their Biochemical and Physiological Roles in Higher Plants.-University of Geneva, Geneva 1982.
    13. Gaspar, T., Penel, C., Castillo, F.J., Greppin, H.: A two step control of basic and acidic peroxidases and its significance for growth and development.-Physiol. Plant. 64: 418-423, 1985. Go to original source...
    14. Goldberg, R., Imberty, A., Chu-Ba, J.: Development of isoperoxidases along the growth gradient in the mung bean hypocotyl.-Phytochemistry 25: 2171-2174, 1986. Go to original source...
    15. Goldberg, R., Kevers, C., Gaspar, T.: Guaiacol and ascorbate peroxidase compartmentation and gradient along the mung bean hypocotyl.-Biochem. Physiol. Pflanz. 184: 155-161, 1989. Go to original source...
    16. Groden, D., Beck, E.: H2O2 destruction by ascorbate dependent systems from chloroplasts.-Biochim. biophys. Acta 546: 426-435, 1979. Go to original source...
    17. Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eichholtz, D., Rogers, S.G., Fraley, R.T.: A simple and general method for transferring genes into plants.-Science 227: 1229-1231, 1985. Go to original source...
    18. Krsnik-Rasol, M.: Peroxidase as a developmental marker in plant tissue culture.-Int. J. Dev. Biol. 35: 259-263, 1991.
    19. Mäder, M., Nessel, A., Bopp, M.: On the physiological significance of the isoenzyme groups of of peroxidase from tobacco demonstrated by biochemical properties. II. pH optima, Michaelis constants and maximal oxidation rates.-Z. Pflanzenphysiol. 82: 247-260, 1977. Go to original source...
    20. Muraja, J., Pe¹kan, T., Lorkoviæ, Z., Krsnik-Rasol, M.: Ultrastructure and protein pattern of horse radish (Armoracia lapathifolia Gilib.) crown-gall tissue.-Acta bot. croat. 53: 1-6, 1994.
    21. Pedreño, M.A., Bernal, M.A., Calderón, A.A., Ferrer, M.A., López-Serrano, M., Merino de Cáceres, F., Muñoz, R., Ros Barceló A.: A general pattern for peroxidase isoenzyme localization and function in Vitaceae, Solanaceae and Leguminoseae.-In: Welinder, K.J., Rasmussen, S.K., Penel, C., Greppin, H. (ed.): Plant Peroxidases: Biochemistry and Physiology. Pp. 307-314. University of Geneva, Geneva 1993.
    22. Rombouts, F.M., Thibault, J.F.: Sugar beet pectins: chemical structure and gelation through oxidative coupling.-In: Fishman, L.M., Jen, J.J. (ed.): Chemistry and Function of Pectins. Pp. 49-60. American Chemical Society, Washington 1986. Go to original source...
    23. Ros Barceló, A., Muñoz, R., Sabater, F.: Lupin peroxidases. I. Isolation and characterization of cell wall bound peroxidase activity.-Physiol. Plant. 71: 448-454, 1987. Go to original source...
    24. Ros Barceló, A., Muñoz, R., Sabater, F.: Lupin peroxidases III. Subcellular localization of membrane-bound acidic isoperoxidases.-Plant Physiol. Biochem. 26: 575-583, 1988.
    25. Ros Barceló, A., Muñoz, R.: Peroxidases: Their role in the control of plant cell growth.-In: Penel, C., Gaspar, T., Greppin, H. (ed.): Plant Peroxidases 1980-1990. Topics and Detailed Literature on Molecular, Biochemical and Physiological Aspects. Pp. 71-80. University of Geneva, Geneva 1992.
    26. Van der Berg, B.M., Chibbar, R.N., Van Huystee, R.B.: A comparative study of a cationic peroxidase from peanut and a anionic peroxidase from Petunia.-Plant Cell Rep. 2: 304-307, 1983. Go to original source...
    27. Zheng, X., Van Huystee, R.B.: Oxidation of tyrosine by peroxidase isoenzymes derived from peanut suspension culture medium and by isolated cell walls.-Plant Cell Tissue Organ. Cult. 25: 35-43, 1991. Go to original source...

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