biologia plantarum

Biologia plantarum 39:581-588, 1997 | DOI: 10.1023/A:1001709405012

Effects of Simulated Acid Rain on Anatomy of Primary Leaves of Phaseolus Vulgaris

D. Stoyanova¹

¹ Department of Botany, Faculty of Biology, Sofia University 'St. Kliment Ohridski', Sofia, Bulgaria

Ten-days-old bean plants (Phaseolus vulgaris L., cv. Cheren Starozagorski) were treated with simulated acid rain (pH 2.4, 2.2, 2.0 and 1.8). Anatomical changes in the primary leaves were studied 3, 48 and 168 h after a single treatment. This treatment induced: 1) change in the shape of palisade cells, contraction of their contact surfaces and expansion of spongy cells (pH 1.8, 3 h after treatment); 2) reduction of symplast connections among palisade cells and of apoplast in the spongy mesophyll (pH 1.8, 48 h after treatment); 3) destruction of adaxial epidermis and portions of palisade mesophyll, plasmolysis of spongy cells (pH 1.8, 168 h after treatment); 4) full destruction of mesophyll (pH 2.4, 2.2, 2.0 and 1.8, 168 h after treatment). The structure of abaxial epidermis was more stable than that of the adaxial one. With respect to anatomical parameters the studied species could be considered as comparatively resistant to acid rain.

Keywords: abaxial and adaxial epidermes; bean; palisade and spongy parenchyma

Subjects: acid rain simulation, leaf anatomy; anatomy, leaf, simulated acid rain; bean, simulated acid rain; epidermis, adaxial, abaxial; parenchyma, palisade and spongy; Phaseolus vulgaris

Published: December 1, 1997  Show citation