biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 38:321, 1996 | DOI: 10.1007/BF02896657

Estimation of ubiquitin and ubiquitin mRNA content in dark senescing wheat leaves

M. L. Pinedo1, S. M. Goicoechea1, L. Lamattina1, R. D. Conde1
1 Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad National de Mar del Plata, Argentina

Wheat leaves(Triticum aestivum L. cv. San Agustin INTA) were detached at the moment they had reached maximum expansion, put in tubes containing water and left in darkness. Under these conditions, leaf protein content decreased mainly as consequence of an increased rate of breakdown. In the range of 0 to 72 h after detachment, western blot analysis of leaf protein extracts displayed both similar proportions of total protein and quality of ubiquitin conjugates. Northern blot analysis of leaf RNA extracts revealed a 1.6 kb ubiquitin mRNA transcript which increased 3.5-fold after 48 h of treatment. Thus wheat leaves maintain both their ability for the ubiquitination of proteins and the transcription of ubiquitin mRNA at stages of senescence in which rates of protein breakdown are increased. The results suggest that the ubiquitin-dependent proteolytic pathway contributes to leaf protein breakdown during senescence

Keywords: in vitro senescence; protein turnover; Triticum aestivum L

Received: May 23, 1995; Accepted: October 19, 1995; Published: September 1, 1996  Show citation

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Pinedo, M.L., Goicoechea, S.M., Lamattina, L., & Conde, R.D. (1996). Estimation of ubiquitin and ubiquitin mRNA content in dark senescing wheat leaves. Biologia plantarum, 38(3), 321. doi: 10.1007/BF02896657
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