biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 38:335-342, 1996 | DOI: 10.1007/BF02896659

Organogenesis andin vitro flowering ofEchinochloa colona. Effect of growth regulators and explant types

P. Das1, S. Samantaray1, G. R. Rout1
1 Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar, India

Echinochloa colona regeneration via organogenesis in callus cultures derived from leaf base and mesocotyl expiants andin vitro flowering were achived. Shoot bud regeneration was achieved on Murashige and Skoog's (MS) basal medium supplemented with 6.66 μM 6-benzylaminopurine (BAP), 2.68 μM 1-naphthalene acetic acid (NAA) and 3 % (m/v) saccharose. Regenerated shoots were rooted on half strength basal MS medium with 2 % (m/v) saccharose devoid of growth regulators. About 90 -95 % of rooted plantlets survived in the greenhouse.In vitro flowering was induced in the regenerated shoots derived from callus on half strength MS medium supplemented with 4.4 μM BAP, 74.07 μM adeninesulphate, 0.72 μM gibberellic acid, and 3 % (m/v) saccharose. The frequency ofin vitro flowering was 80 - 90 % in three repeated experiments. Fertile seeds were recovered fromin vitro grown plantlets which were subsequently germinated into plants.

Keywords: metal tolerance; regeneration

Received: August 9, 1995; Accepted: January 15, 1996; Published: September 1, 1996  Show citation

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Das, P., Samantaray, S., & Rout, G.R. (1996). Organogenesis andin vitro flowering ofEchinochloa colona. Effect of growth regulators and explant types. Biologia plantarum, 38(3), 335-342. doi: 10.1007/BF02896659
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