biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:523, 1995 | DOI: 10.1007/BF02908832

Organ-specific expression of the stress-related anionic peroxidases in cucumber flowers

V. Repka1, M. Jung1
1 Laboratory of Molecular Biology and Virology, CRIVE, Bratislava, Slovakia

The study revealed a marked qualitative and quantitative differences in the pattern of expression of three stress-related cucumber (Cucumis sativus L., cv. Laura) isoperoxidases. Activity staining, as well as the protein gel blot (Western blotting) confirmed that the proteins studied are differentially expressed in both male and female sepals, in male but not in female pedicels and in, pistil. By using antibodies specific to three stress-related peroxidases, one serologically related pistil-specific anionic peroxidase was detected. This specific band had never been observed in other flower organs. Differential appearance of the stress-related peroxidase isoenzymes in both male and female flowers demonstrates that these proteins are developmentally regulated, showing an organ-specific expression.

Keywords: Cucumis; ovary; pedicel; petal; pistil; sepal; stamen; tobacco necrosis virus

Received: March 24, 1995; Accepted: June 13, 1995; Published: December 1, 1995  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Repka, V., & Jung, M. (1995). Organ-specific expression of the stress-related anionic peroxidases in cucumber flowers. Biologia plantarum, 37(4), 523. doi: 10.1007/BF02908832
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bacic, A., Gell, A.C., Clarke, A.E.: Arabinogalactan proteins from stigmas ofNicotiana alata- Phytochemistry27: 679-684, 1988. Go to original source...
    2. Bernal, M.A., Pedreño, M.A., Calderón, A.A., Muñoz, R., Ros Barceló, A., Merino de Cáceres, F.: The subcellular localization of isoperoxidases inCapsicum annum leaves and their different expression in vegetative and flowering plants.-Ann. Bot.72: 415-421, 1993. Go to original source...
    3. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding.-Anal. Biochem.72: 248-254, 1976. Go to original source...
    4. Chibbar, R.N., Van Huystee, R.B.: Characterization of peroxidase in plants.-Plant Physiol75: 956-958, 1984. Go to original source...
    5. Dalet, F., Cornu D.: Lignification level and peroxidase activity duringin vitro rooting ofPrunus avium.-Can. J. Bot67: 2182-2186, 1989. Go to original source...
    6. Dunford, H.B.: Horseradish peroxidase: structure and kinetic properties.-In: Everse, J., Grisham, M.B. (ed.): Peroxidase Chemistry and Biology. Vol. II. Pp.1-24. CRC Press, Boca Raton 1991.
    7. Felix, G., Meins, F.: Developmental and hormonal regulation of β-1,3-glucanase in tobacco.-Planta167: 206-211, 1986. Go to original source...
    8. Frankel, R., Galun, E. Pollination Mechanisms, Reproduction, and Plant Breeding.-Springer-Verlag, Heidelberg-Berlin-New York 1977. Go to original source...
    9. Frič, F., Fuchs W.H.: Veräderungen der Aktivität einiger Enzyme in Weizenblatt in Abhängigkeit von der temperaturlabilen Verträglichkeit fürPuccinia graminis tritici.-Phytopathol. Z.67: 161-174, 1970. Go to original source...
    10. Gaspar, T., Penel, C., Thorpe, T., Greppin, H.: Peroxidases: A Survey of Their Biochemical and Physiological Roles in Higher Plants.-University of Geneva, Geneva 1982.
    11. Gaspar, T., Kevers, C., Hausman, J.F., Berthon, J.Y, Ripetti, V.: Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots.-Agronomie12: 757-765, 1992. Go to original source...
    12. Goldman, M.H.de S., Pezzotti, M., Seurinck, J., Mariani, C.: Developmental expression of tobacco pistil-specific genes encoding novel extensin-like proteins.-Plant Cell4: 1041-1051, 1992. Go to original source...
    13. Greppin, H., Penel, C., Gaspar, T.: Molecular and Physiological Aspects of Plant Peroxidases.- University of Geneva, Geneva 1986.
    14. Hirsch, A.M., Fortune, D.: Peroxidase activity and isoperoxidase composition in cultured stem tissue, callus and cell suspensions ofActinidia chinensis-Z. Pflanzenphysiol113: 129-139, 1984. Go to original source...
    15. Hong, J.C., Nagao, R.T., Key, J.L.: Developmentally regulated expression of, soybean proline-rich cell wall protein genes.-Plant Cell1: 937-943, 1989. Go to original source...
    16. Kahlem, G.: A specific and general biochemical marker of stamen morphogenesis in higher plants: Anodic peroxidases.-Z. Pflanzenphysiol76: 80-85, 1975. Go to original source...
    17. Keil M., Sanchez-Serrano, J., Willmitzer, L.: Both wound-inducible and tuber-specific expression are mediated by the promoter of a single member of the potato proteinase inhibitor II gene family.- EMBO J8: 1323-1330, 1989. Go to original source...
    18. Lotan, T., Ori, N., Fluhr, R.: Pathogenesis-related proteins are developmentally regulated in tobacco flowers.-Plant Cell1: 881-887, 1989. Go to original source...
    19. Medina, M.I., De Forchetti, S.M., Tigier, H.A.: Kinetic properties of alfalfa root isoperoxidases with IAA oxidase and syringaldazine oxidase activities.-In: Welinder, K.G., Rasmussen, S.K., Penel, C., Greppin, H. (ed.): Plant Peroxidases. Biochemistry and Physiology. Pp.175-180 University of Geneva, Geneva, 1993.
    20. Memelink, J., Linthorst H.J., Schilperoort R.A., Hoge, H.J.C.: Tobacco genes encoding acidic and basic isoforms of pathogenesis-related proteins display different expression patterns.-Plant mol. Biol.14: 119-126, 1990. Go to original source...
    21. Nakanishi, F., Fujii, T.: Appearance of peroxidase isozymes in floral-initiated shoot apices ofPharbitis nil-Physiol Plant86: 197-201, 1992. Go to original source...
    22. Penel, C., Gaspar, T., Greppin, H.: Plant peroxidases 1980-1990. Topics and Detailed Literature on Molecular, Biochemical, and Physiological Aspects.-University of Geneva, Geneva 1992.
    23. Repka, V., Kollerová, E., Slovák, M.: The extracellular acidic and basic virus-elicited proteins of cucumber cotyledons.-Biol. Plant35, 87-93, 1993. Go to original source...
    24. Repka, V., Slováková, Ĺ: Purification, characterization and accumulation of three virus-induced cucumber peroxidases.-Biol Plant.36: 121-132, 1994. Go to original source...
    25. Repka, V., Slováková, Ĺ., Kollerová, E., Slovák, M.: [Association of the enzymatic activity with expression of PR-proteins in leaves ofCucumis sativusL. infected by tobacco necrosis virus.]-In: X. National Genetic Conference Pp. 191-192. Agricultural University, Brno 1991. [In Slovak]
    26. Repka, V., Vanek, G.: Characterization of the stress-related, extracellular anionic peroxidases from cucumber.-In: Welinder, K.G., Rasmussen, S.K., Penel, C., Greppin, H. (ed.). Plant Peroxidases: Biochemistry and Physiology. Pp. 355-360. University of Geneva, Geneva 1993.
    27. Ros Barceló, A., Muñoz, R.: Peroxidases: their role in the control of plant cell growth.-In: Greppin, H., Penel, C., Gaspar, T. (ed.): Peroxidases 1980-1990. Progress and Prospects in Biochemistry and Physiology. Pp. 71-89. University of Geneva, Geneva 1992.
    28. Van Huystee, R.B.: Some molecular aspects of plant peroxidase biosynthetic studies.-Annu. Rev. Plant Physiol.38: 205-217, 1987. Go to original source...
    29. Welinder, K.G., Rasmussen, S.K., Penel, C., Greppin, H.: Plant Peroxidases: Biochemistry and Physiology.-University of Geneva, Geneva 1993.
    30. Ye, Z.-H., Varner, J.E.: Tissue-specific expression of cell wall proteins in developing soybean tissues.-Plant Cell3: 23-37, 1991. Go to original source...
    31. Zhou, X., Han, Y., Yang, W., Xi, T.: Somatic embryogenesis and analysis of peroxidase in cultured lettuce (Lactuca sativa L.) cotyledons.-Ann. Bot.69: 97-100, 1992. Go to original source...

    Return to the content