biologia plantarum

International journal on Plant Life established by Bohumil Nģmec in 1959

Biologia plantarum 37:541, 1995 | DOI: 10.1007/BF02908836

The effects of lead and kinetin on greening barley leaves

A. Wo¼ny1, J. Schneider1, E. A. Gwó¼d¼1
1 Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, Poland

The content of lead in greening etiolated barley leaves remained the same, regardless the time of incubation of excised leaves in the presence of lead ions (8-24 h). The lead deposits have not been detected within mesophyll cells, but were found in intercellular spaces of mesophyll, in guard cells and in cuticle covering stomata. This suggests that lead may be transported in the leavesvia transpiration stream. Lead reduced the content of chlorophyll, especially chlorophyllb content and the average number of grana, whereas in the presence of kinetin the content of chlorophyll increased. In the combined treatment (lead + kinetin) kinetin diminished the inhibitory effect of lead on the chlorophyll content. The number of chloroplasts in mesophyll cells remained unchanged after lead treatment, whereas kinetin alone or applied together with lead increased the average chloroplasts number. The thylakoids system in chloroplasts of kinetin and kinetin + lead treated plants was similar to that observed in control, although the grana number was smaller. Both lead and kinetin increased the content of condensed chromatin in nuclei.

Keywords: chlorophyll; chloroplast; growth regulators; heavy metal; Hordeum vulgare; ultrastructure

Received: December 19, 1994; Accepted: April 11, 1995; Published: December 1, 1995  Show citation

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Wo¼ny, A., Schneider, J., & Gwó¼d¼, E.A. (1995). The effects of lead and kinetin on greening barley leaves. Biologia plantarum, 37(4), 541. doi: 10.1007/BF02908836
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    References

    1. Arnon, D.J.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris.-Plant Physiol.24: 1-15, 1949. Go to original source...
    2. Bazzaz, F.A., Carlson, R.W., Rolfe, G.L.: The effect of heavy metals on plants: Part I. Inhibition of gas exhange in sunflower by Pb, Cd, Ni and Tl.-Environ. Pollut.7: 241-246, 1974. Go to original source...
    3. Bazzaz, F.A., Carlson, R.W., Rolfe, G.L.. Inhibition of corn and sunflower photosynthesis by lead.- Physiol. Plant.34: 326-329, 1975. Go to original source...
    4. Bazzaz, F.A., Govindjee: Effects of lead chloride on chloroplast reaction.-Environ. Lett.6: 175-191, 1974. Go to original source...
    5. Becerril, J.M., Muñoz-Rueda, A., Aparicio-Tejo, P., Gonzalez-Murua, C.: The effects of cadmium and lead on photosynthetic electron transport in clover and lucerne.-Plant Physiol. Biochem.26: 357-363, 1988.
    6. Bessonova, V.P., Lyzhenko, I.I., Mikhailov, O.F., Kulaeva, O.N. [Influence of cytokinin on plant growth and chlorophyll content in leaves under conditions of air pollution.]-Fiziol. Rast.31: 1149-1153, 1984. [In Russ.]
    7. Burzyński, M.: Influence of lead on the chlorophyll content and on initial steps of its synthesis in greening cucumber seedlings.-Acta Soc. Bot. Pol.54: 95-107, 1985. Go to original source...
    8. Burzyński, M.: [Influence of lead on physiological processes of plant].-Wiad. Bot.31: 87-96, 1987. [In Pol.]
    9. Čiamporovį, M.: Recovery of ultrastructure in water stressed root epidermal cells ofZea mays.-In: Loughman, B.C., Ga¹parķkovį, O., Kolek, J. (ed.): Structural and Functional Aspects of Transport in Roots. Pp. 263-267. Kluwer Academic Publishers, Dordrecht-Boston-London 1989. Go to original source...
    10. Glater, R.A., Hernandez, L.: Lead detection in living plant tissue using a new histochemical method.- J. Air Pollut. Control Assoc.22: 463-467, 1972. Go to original source...
    11. Hiscox, J.D., Israelstam, G.F.: A method for the extraction of chlorophyll from leaf tissue without maceration.-Can. J. Bot.57: 1332-1334, 1979. Go to original source...
    12. Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy.-J. Cell Biol.27: 137-138, 1965.
    13. Kinoshita, I., Sanbe, A., Yokomura, E.-I.: Increaces in nuclear DNA content without mitosis in benzyladenine-treated primary leaves of intact and decapitated bean plants.-J. exp. Bot.42: 667-672, 1991. Go to original source...
    14. Miles, C.D., Brandle, J.R., Daniel, D.J., Chu-Der, O., Schnare, P.D., Uhlik, D.J.: Inhibition of photosystem II in isolated chloroplasts by lead.-Plant Physiol.49: 820-825, 1972. Go to original source...
    15. Nagl, W., Peschke, W.: Automatische Diagnose der artspezifischen Kern-Ultrastructur sowie von Zellzyklusstadien bei Pflanzen mit Hilfe des Bildanalysesystems Leitz T.A.S.-Leitz-Mitt. Wiss. Technol.8: 29-32, 1982.
    16. Poskuta, J.W., Parys, E., Romanowska, E.: The effects of lead on the gaseous exchange and photosynthetic carbon metabolism of pea seedlings.-Acta Soc. Bot. Pol.56: 127-137, 1987. Go to original source...
    17. Poskuta, J.W., Parys, E., Romanowska, E., Gajdzis-Gujdan, H., Wróblewska, B.: The effects of lead on photosynthesis,14C distribution among photoassimilates and transpiration of maize seedlings. -Acta Soc. Bot. Pol.57: 149-155, 1988. Go to original source...
    18. Radecki, J., Banaszkiewicz, T., Klasa, A.: The effect of different lead compounds on mitotic activity of maize root tip cells.-Acta Physiol. Plant.11: 125-130, 1989.
    19. Sharma, S.D., Chopra, R.N.: Effect of lead nitrate and lead acetate on growth of the mossSemibarbula orientalis (Web.) Wijk.et Marg. grownin vitro.-J. Plant Physiol.28: 243-249, 1987. Go to original source...
    20. Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy.-J. Ultrastruct. Res.26: 31-43, 1969. Go to original source...
    21. Stiborovį, M., Ditrichovį, M., Bųezinovį, A.: Effect of heavy metal ions on growth and biochemical characteristics of photosynthesis of barley and maize seedlings.-Biol. Plant.29: 453-467, 1987. Go to original source...
    22. Wellburn, A.R., Gounaris, I., Fässler, L., Lichtenthaler, H.K.: Changes in plastid ultrastructure and fluctuations of cellular isoprenoid and carbohydrate compounds during continued etiolation of dark-grown oat seedlings.-Physiol. Plant.59: 347-354, 1983. Go to original source...
    23. Wellburn, A.R., Hampp, R.: Appearance of photochemical function in prothylakoids during plasid development.-Biochem. biophys. Acta547: 380-397, 1979. Go to original source...
    24. Wierzbicka, M.: Lead translocation and localization inAllium cepa roots.-Can. J. Bot.65: 1851-1860, 1987. Go to original source...
    25. Wong, D., Govindjee: Effects of lead ions on photosystem I in isolated chloroplasts: studies on the reaction center P700.-Photosynthetica10: 241-254, 1976.
    26. Wo¼ny, A.: Toxic Effect of Lead Compounds on Early Stages of Plant Development.-Wydawnictwo Naukowe UAM, Poznañ 1987.
    27. Wo¼ny, A., Jerczyńska, E.: The effect on lead on early stages ofPhaseolus vulgaris L. growth inin vitro conditions.-Biol. Plant.33: 32-39, 1991. Go to original source...
    28. Wrischer, M., Meglaj, D.: The effect of lead on the structure and function of wheat plastids.-Acta bot. croat.39: 33-40, 1980.

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