biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:169, 1995 | DOI: 10.1007/BF02913205

Induction, protein composition and DNA ploidy level of embryogenic calli of silver fir and its hybrids

A. Gajdošová1, B. Vooková1, A. Kormuťák1, G. Libiaková1, J. Doležel2
1 Institute of Plant Genetics, Slovak Academy of Sciences, Nitra, Slovakia
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

Somatic embryogenesis was initiated from immature zygotic embryos of intraspecific cross ofAbies alba and interspecific combination ofAbies alba x Abies nordmanniana. The most responsive explants for induction of embryogenic calli were zygotic embryos in precotyledonary stage of development. Biochemically the compared embryogenic lines were uniform irrespective of their morphology or embryogenic potential. The flow cytometric analysis of single embryogenic lines suggests that no changes in ploidy levels occur during induction and culture of embryogenic callus inAbies alba what confirms the convenience of this system for propagation of valuable seed material.

Keywords: Abies alba; Abies nordmanniana; flow cytometry; somatic embryogenesis

Received: June 20, 1994; Accepted: August 25, 1994; Published: June 1, 1995  Show citation

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Gajdošová, A., Vooková, B., Kormuťák, A., Libiaková, G., & Doležel, J. (1995). Induction, protein composition and DNA ploidy level of embryogenic calli of silver fir and its hybrids. Biologia plantarum, 37(2), 169. doi: 10.1007/BF02913205
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