biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 37:355, 1995 | DOI: 10.1007/BF02913978

Growth,14CO2 fixation, activites of photosystems, ribulose 1,5-bisphosphate carboxylase and nitrate reductase in trees as affected by simulated acid rain

K. Muthuchelian1, C. Murugan1, R. Harigovindan1, N. Nedunchezhian2, G. Kulandaivelu2
1 School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai, India
2 School of Biological Sciences, Madurai Kamaraj University, Madurai, India

In seedlings of the tropical tree speciesErythrina variegata Lam. andHardwickia binata Roxb. exposed to different acidic mist (H2SO4, pH 5, 3 and 2) for 5 d significant reduction in seedling growth, biomass accumulation and14CO2 fixation were determined. In isolated chloroplasts a decrease in the activities of photosystem 2 and whole electron transport chain was observed only at pH 3 and 2, but no significant change in photosystem 1 activity was observed. SDS-PAGE analysis of crude leaf extracts of ribulose 1,5-bisphosphate carboxylase (RuBPC) indicated a significant loss of 55 and 15 kDa polypeptides at pH 2 inErythrina. The reduction in the RuBPC activity in seedlings grown under acidic mists correlated well with CO2 fixation.

Keywords: Erythrina variegata; Hardwickia binata; H2SO4; polypeptides; photosynthesis

Received: August 1, 1994; Accepted: October 17, 1994; Published: September 1, 1995  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Muthuchelian, K., Murugan, C., Harigovindan, R., Nedunchezhian, N., & Kulandaivelu, G. (1995). Growth,14CO2 fixation, activites of photosystems, ribulose 1,5-bisphosphate carboxylase and nitrate reductase in trees as affected by simulated acid rain. Biologia plantarum, 37(3), 355. doi: 10.1007/BF02913978
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris.-Plant Physiol.24: 1-15, 1949. Go to original source...
    2. Allen, L.H., Jr., Vu, C.V., Berg, R.H., Garrard, L.A.: Impact of solar UV-B radiation on crops and crop canopies.-In: UV-B Biological and Climatic Effects Research. Final Report. USDA/EPA, Washington 1978.
    3. Armond, P.A., Schreiber, U., Björkman, O.: Photosynthetic acclimation to temperature in the desert shrubLarrea divaricata. II. Light harvesting efficiency and electron transport.-Plant Physiol.61: 411-415, 1978. Go to original source...
    4. Bowes, G., Ogren, W. L.: Oxygen inhibition and other properties soybean ribulose 1,5-diphosphate carboxylase.-J. biol. Chem.247: 2171-2176, 1972. Go to original source...
    5. Chappelka, A.H., Chevone, B.I., Seiler, J.R.: Growth and physiological responses of yellow-poplar seedlings exposed to ozone and simulated acidic rain.-Environ. Pollut.49: 1-18, 1988. Go to original source...
    6. Chen, Y.M., Lucas, P.W., Wellburn, A.R.: Relationship between foliar injury and changes in antioxidant levels in red and Norway spruce exposed to acidic mists.-Environ. Pollut.69: 1-15, 1991. Go to original source...
    7. Dubois, M., Gilles, K.N., Hamilton, J.K., Robers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances.-Anal. Chem.28: 350-356, 1956. Go to original source...
    8. Ellis, J.: Chloroplasts proteins: synthesis, transport, and assembly.-Annu. Rev. Plant Physiol.32: 117-137, 1981. Go to original source...
    9. Franzen, J., Gross, K., Bonzon, M., Wagner, E.: Light quality and quantity effects on monoterpene mediated photo-bleaching of conifer needles.-Ann. Sci. forest.46 (Suppl.):565-567, 1989. Go to original source...
    10. Goodwin, T.W.: Carotenoids.-In: Paech, K., Tracey, M.W. (ed.). Handbook of Plant Analysis. Vol. III. Pp. 272-311. Springer-Verlag, Berlin 1954. Go to original source...
    11. Jaworski, E.G.: Nitrate reductase assay in intact plant tissues.-Biochem. biophys. Res. Commun.43: 1247-1279, 1971. Go to original source...
    12. Kulandaivelu, G., Daniell, H.: Dichlorophenyl dimethyl urea (DCMU) induced increase in chlorophyll a fluorescence intensity. An index of photosynthetic O2 evolution in leaves, chloroplasts and algae. -Physiol. Plant.48: 385-388, 1980. Go to original source...
    13. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriphage-T4. -Nature227: 680-685, 1970. Go to original source...
    14. Lee, E.S., Chevone, B.I., Seiler, J.R.: Growth response and drought susceptibility of red spruce seedlings exposed to simulated acidic rain and ozone.-Forest Sci.36: 265-275, 1990.
    15. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem.193: 265-275, 1951. Go to original source...
    16. Martens, K.C., Landuydt, K., Impens, I.: Direct effects of acidic wet deposition on photosynthesis and stomatal conductance of twoPopulus clones (P. cv. Beaupre andP. cv. Robusta.).-Ann. Sci. forest.46 (Suppl.): 586-589, 1989. Go to original source...
    17. McCready, F.M., Guggolz, J., Silivera, V., Owen, H.S.: Determination of starch and amylose in vegetables.-Anal. Chem.52: 1156, 1950. Go to original source...
    18. Muthuchelian, K., Nedunchezhian, N., Kulandaivelu, G.: Effect of simulated acid rain on14CO2 fixation, ribulose-1,5 bisphosphate carboxylase and nitrate and nitrite reductases inVigna sinensis andPhaseolus mungo.-Photosynthetica28: 361-367, 1993.
    19. Muthuchelian, K., Nedunchezhian, N., Kulandaivelu, G.: Acid rain: Acidic mist induced response in growth and photosynthetic activities on crop plants.-Arch. Environ. Contam. Toxicol.26: 521-526, 1994. Go to original source...
    20. Muthuchelian, K., Paliwal, K., Gnanam, A.: The effect of three long-chain aliphatic alcohols on photosynthesis and growth ofPennisetum polystachyeon Schult.- Photosynthetica24: 257-260, 1990.
    21. Noorudeen, A.M., Kulandaivelu, G.: On the possible site of inhibition of photosynthetic electron transport by ultraviolet-B (UV-B) radiation.-Physiol. Plant.55: 161-166, 1982. Go to original source...
    22. Radford, P.J.: Growth analysis formulae-their use and abuse.-Crop Sci.7: 171-175, 1967. Go to original source...
    23. Reeves, S.G., Hall, D.O.: The stoichiometry (ATP/2e ratio) of non-cyclic photophosphorylation in isolated spinach chloroplasts.-Biochim. biophys. Acta314: 66-78, 1973. Go to original source...
    24. Sasek, T.W., Richandson, C.J., Fendick, E.A., Bevington, S.R., Kress, L.W.: Carryover effects of acid rain and ozone on the physiology of multiple flushes pine seedlings.-Forest Sci.37: 1078-1098, 1991.
    25. Saxe, H.: Photosynthesis and stomatal responses to polluted air and the use of physiological and biochemical responses for early detection and diagonostic tools.-Adv. bot. Res.18: 1-128, 1991. Go to original source...
    26. Scherbatskoy, T.: Ionic interactions between precipitation and leaf cuticles.-Ann. Sci. forest.46 (Suppl.): 149-157, 1989. Go to original source...
    27. Smith, C.R., Vasilas, B.L., Banwart, W.L., Peters, D.B., Walker, W.M.: Lack of physiological response of two corn hybrids to simulated acid rain.-Environ. exp. Bot.3: 435-442, 1990. Go to original source...
    28. Takemoto, B.K., Shriner, D.S., Hohnston, I.W.: Physiological responses of soybean to stimulated acid rain and ambient ozone in the field.-Water Air Soil Pollut.33: 373-384, 1987. Go to original source...
    29. Van Elsacker, P., Martens, C., Impens, I.: Direct effects of acid wet deposition on photosynthesis, stomatal conductance and growth ofPopulus cv. Beaupre.-In: Mathy, P. (ed.): Air Pollution and Ecosystems. Pp. 728-733. D. Reidel Publ. Co., Dordrecht 1989. Go to original source...
    30. Van Elsacker, P., Impens, I.: Response of shoot growth and gas exchange ofPicea abies clones to rain acidity and the addition of ions.-Ann. Sci. forest.46 (Suppl.): 595-598, 1988. Go to original source...
    31. Weigel, H.J., Halbwachs, G., Jager, H.G.: The effects of air pollutants on forest trees from a plant physiological view.-Z. Pflanzenkrankh. Pflanzenschutz96: 203-217, 1989.
    32. Wellburn, A.R.: Air Pollution and Acid Rain: The Biological Impact.-Longman Scientific and Technical, London 1988.

    Return to the content