biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 36:373, 1994 | DOI: 10.1007/BF02920935

Interactive effect of heavy metals and temperature on the growth, and chlorophyll, saccharides and soluble nitrogen contents inPhaseolus plants

M. A. A. Gadallah1
1 Botany Department, Faculty of Science, Assiut University, Assiut, Egypt

The effect of combinations of different concentrations of copper (Cu) and mercury (Hg) ions with different constant temperatures (T) onPhaseolus vulgaris plants was studied. Criteria investigated included shoot and root length, dry mass, chlorophyll content (Chl) and stability index (CSI), and contents of soluble (SS) and hydrolysable saccharides (HS), soluble proteins (SP) and total free amino acid (AA). Each of the factors (T, Cu and Hg) significantly affected the parameters tested with exception of T effect on Chlb content as well as on Chla/b ratio. Bifactorial interactions (T×Cu) or (T×Hg) were also significant, except the interaction (T×Hg) in shoot elongation, Chlb content and Chla/b ratio. Statistical treatment of the data lead to three findings: (1) temperature was dominant in affecting CSIa, shoot AA and root SS, (2) Cu and Hg had the predominant effect on growth parameters and Chl content, and (3) interactions (T×Cu and T×Hg) were dominant in affecting CSIb, shoot SP, and root HS.

Received: November 22, 1993; Accepted: February 18, 1994; Published: September 1, 1994  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Gadallah, M.A.A. (1994). Interactive effect of heavy metals and temperature on the growth, and chlorophyll, saccharides and soluble nitrogen contents inPhaseolus plants. Biologia plantarum, 36(3), 373. doi: 10.1007/BF02920935
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ahmed, I., Hellebust, J.A.: The relationship between inorganic nitrogen metabolism and proline accumulation in osmoregulatory responses of two euryhaline microalgae.-Plant Plant Physiol.88: 348-354, 1988. Go to original source...
    2. Alia, Pardha Saradhi, P.: Proline accumulation under heavy metal stress.-J. Plant Physiol.138: 554-558, 1991. Go to original source...
    3. Clijsters, H., Van Assche, F.: Inhibition of photosynthesis by heavy metals.-Photosynth. Res.7: 31-40, 1985. Go to original source...
    4. De Filippis, L.F.: The effect of heavy metal compounds on the permeability ofChlorella cells.-Z. Pflanzenphysiol.92: 39-49, 1979. Go to original source...
    5. Dubois, M., Gilles, K.A., Hamilton, J.K., Rabers, R.A., Smith, F.: Colorimetric method for the determination of sugars and related substances.-Anal. Chem.28: 350-356, 1956. Go to original source...
    6. El-Sharkawi, H.M., Springuel, I.V.: Germination of some crop plant seeds under reduced water potential.-Seed Sci. Technol.5: 677-688, 1977.
    7. Fukutaku, Y., Yamada, Y.: Source of proline nitrogen in water stressed soybean (Glycine max) II. Fate of15N-labelled protein.-Physiol. Plant.61: 622-628, 1984. Go to original source...
    8. Hay, R.K.M., Walker, A.L.: An Introduction to the Physiology of Crop Yield.-Longman Scientific and Technical, Harlow 1989.
    9. Kadpal, R.P., Rao, N.A.: Alterations in the biosynthesis of proteins and nucleic acids in finger millet (Eleucine coracana) seedling during water stress and the effect of proline on protein biosynthesis. -Plant Sci.40: 73-79, 1985. Go to original source...
    10. Laliberte, G., Hellebust, J.A.: Regulation of proline content ofChlorella autotropica in response to change in salinity.-Can. J. Bot.67: 1959-1965, 1989. Go to original source...
    11. Lee, Y.P., Takahanshi, T.: An improved colorimetric determination of amino acids with the use of ninhydrine.-Anal. Biochem.14: 71-77, 1966. Go to original source...
    12. Levitt, J.: Responses of Plant to Environmental Stresses. Vol. 1.-Academic Press, New York 1980.
    13. Lidon, F.C., Henriques, F.S.: Effects of copper on the nitrate to ammonia reduction mechanism in rice plants.-Photosynthetica26: 371-380, 1992.
    14. Lidon, F.C., Henriques, F.S.: Changes in the contents of the photosynthetic electron carriers, RNase activity and membrane permeability, triggered by excess copper in rice.-Photosynthetica28: 99-108, 1993.
    15. Long, SP, Woodward, F.I (ed.): Plants and Temperature.-Company of Biologists, Cambridge 1988.
    16. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem.193: 265-275, 1951. Go to original source...
    17. Mohanty, N., Mohanty, P.: Cation effects on primary processes of photosynthesis.-In: Singh, R., Sawhney, S.K. (ed.): Advances in Frontier Areas of Plant Biochemistry, Pp. 1-18. Prentice-Hall, New Delhi 1988.
    18. Murthy, S.D.S., Bukhov, N.G., Mohanty, P.: Mercury-induced alterations of chlorophylla fluorescence kinetics in cyanobacteria: multiple effects of mercury on electron transport.-J. Photochem. Photobiol.6: 373-380, 1990. Go to original source...
    19. Murty, K.S., Majumder, S.K.: Modification of technique for determination of chlorophyll stability index in relation to studies of drought resistance in rice.-Curr. Sci.31: 470-471, 1962.
    20. Nath, R.: Biochemical effects.-In: Bhatia, B. (ed.): Environmental Pollution of Cadmium, Biological Physiological and Health Effects. Pp. 60-110, Interprint, New Delhi 1986.
    21. Nikolopoulos, D., Manetas, Y.: Compatible solutes andin vitro stability ofSalsola soda enzymes: proline incompatibility.-Phytochemistry30: 411-413, 1991. Go to original source...
    22. Ohsumi, Y., Kitamoto, K., Anraku, Y.: Changes induced in the permeability barrier of the yeast plasma mambrane by cuperic ion.-J. Bacteriol.170: 2676-2682, 1988. Go to original source...
    23. Ostle, B.: Statistics in Research.-Iowa State University Press, Ames 1963.
    24. Påhlsson, A.M.B.: Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants.-Water Air Soil Pollut.47: 287-319, 1989. Go to original source...
    25. Paleg, L., Stewart, G.R. and Bradbeer, J.W.: Proline and glycine betaine influences protein solvation. -Plant Physiol.75: 974-978, 1984. Go to original source...
    26. Passow, H., Rothstein, A., Clarkson, T.W.: The general pharmacology of the heavy metals.- Pharmacol. Rev.13: 185-224, 1961.
    27. Pucher, W.G., Leavensworth, C.S., Vickery, H.B.: Determination of starch in plant tissues.-Anal. Chem.20: 850-853, 1948. Go to original source...
    28. Stiborová, M., Ditrichová, M., Bøezinová, A.: Effect of heavy metal ions on growth and biochemical characteristics of photosynthesis of barley and maize seedlings.-Biol. Plant.29: 453-467, 1987. Go to original source...
    29. Stobart, A.K., Griffiths, W.T., Ameen-Bukhari, I., Sherwood, R.P.: The effect of Cd on the biosynthesis of chlorophyll in leaves of barley.-Physiol. Plant.63: 293-298, 1985. Go to original source...
    30. Stoyanova, D.P., Tchakalova, E.S.: The effect of lead and copper on the photosynthetic apparatus inElodea canadensis Rich.-Photosynthetica28: 63-74, 1993.
    31. Todd, G.W., Basler, E.: Fate of various protoplasmic constituents in droughted wheat plants.-Phyton22: 79-85, 1965.
    32. Van Asshe, F., Clijsters, H.: Effects of metals on enzyme activity in plants.-Plant Cell Environ.13: 195-206, 1990. Go to original source...
    33. Van Steveninck, R.F.M., Van Steveninck, M.E., Wells, A.J., Fernando, D.R.: Zinc tolerance and the binding of zinc as zinc phytate inLemna minor. X-ray microanalytical evidence.-J. Plant Physiol.137: 140-146, 1990. Go to original source...
    34. Wainwright, S.J., Woolhouse, H.W.: Some physiological aspects of copper and zinc tolerance inAgrostis tenuis Sibth.: Cell elongation and membrane damage.-J. exp. Bot.28: 1029-1036, 1977. Go to original source...
    35. Woodward, F.I. Climate and Plant Distribution.-Cambridge University Press, Cambridge 1987.

    Return to the content