biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 36:161, 1994

Constitutive expression of extracellular peroxidase isoenzymes capable of oxidizing 4-hydroxystilbenes during the growth cycle of grapevine suspension cell cultures

A. A. Calderón1, J. M. Zapata1, M. A. Pedreño1, A. Ros Barceló1
1 Department of Plant Biology (Plant Physiology), University of Murcia, Murcia, Spain

The constitutive expression of peroxidase isoenzymes which are capable of oxidizing 4-hydroxystilbenes was studied during the growth cycle of suspension cell cultures from grapevine (Vitis vinifera L. cv. Monastrell) berries. the results showed that the growth of suspension cell cultures is accompanied by the constitutive expression of the peroxidase isoenzymes HSPrx 1 and HSPrx 2, previously characterised by their properties for oxidizing 4-hydroxystilbene, the expression of these isoenzymes being characteristic of Monastrell vines brought to a resistant state. However, although the differential catalytic properties of these grapevine isoenzymes are also shown by the horseradish peroxidase, no immunological relationships have been found between the peroxidases from either source.

Received: May 25, 1993; Accepted: June 17, 1993; Published: June 1, 1994  Show citation

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Calderón, A.A., Zapata, J.M., Pedreño, M.A., & Ros Barceló, A. (1994). Constitutive expression of extracellular peroxidase isoenzymes capable of oxidizing 4-hydroxystilbenes during the growth cycle of grapevine suspension cell cultures. Biologia plantarum, 36(2), 161
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