biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 36:167-173, 1994

Effect of growth regulators onin vitro propagation ofFicus benjamina cv. Exotica

J. B. del Amo-Marco1, I. Picazo1
1 Departamento de Biologia Vegetal, Universitat de Valencia, Valencia, Spain

Stem internodes with axillary buds were excised from 5-year old trees ofFicus benjamina cv. Exotica. The effect of 6-benzylaminopurine (BAP), gibberellic acid (GA3), indole-3-acetic acid (IAA), naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D) on shoot growth and proliferationin vitro was investigated. Multiple shoots were developed after 3-4 weeks from stem internodes with axillary buds incubated in Murashige and Skoog (MS) medium supplemented with phloroglucinol (PG) and BAP. Optimum shoot proliferation took place in the presence of 1.0 mg l-1 BAP. Shoots obtained could be elongated in a medium with 0.5 mg l-1 GA3 prior to their rooting. The root initiation was successfully induced on MS medium either with IAA at 0.5-0.1 mg l-1 or in plant growth regulator-free medium. All rooted plantlets were subsequently transferred to a peat, humus and perlite mixture in a culture room with high humidity and covered with plastic bags. After one month the plantlets were established for growing in a greenhouse.

Received: July 16, 1993; Accepted: September 3, 1993; Published: June 1, 1994  Show citation

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del Amo-Marco, J.B., & Picazo, I. (1994). Effect of growth regulators onin vitro propagation ofFicus benjamina cv. Exotica. Biologia plantarum, 36(2), 167-173
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