biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 36:511-517, 1994 | DOI: 10.1007/BF02921170

Alleviation of browning in oak explants by chemical pretreatments

K. Tóth1, T. Haapala2, A. Hohtola2
1 Szombathely State Forestry, Szombathely, Hungary
2 Department of Botany, University of Oulu, Oulu, Finland

Meristems from 25-90-year-old oak (Quercus robur L. andQ. petraea Matt.) trees and seed embryos were pretreated with polyvinyl pyrrolidone, ascorbic acid, cysteine and citric acid solutions. Tissues were cultured mostly on a WPM medium supplemented with different combinations and concentrations of growth regulators. All the different pretreatments showed a positive effect against the otherwise very rapid and harmful browning of the explants but ascorbic acid (100 mg dm-3) proved to be the most effective. Shooting was induced from seed embryos and meristems originating from adult trees. Rooted plantlets were obtained from explants of seed embryos.

Received: November 5, 1993; Accepted: May 2, 1994; Published: December 1, 1994  Show citation

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Tóth, K., Haapala, T., & Hohtola, A. (1994). Alleviation of browning in oak explants by chemical pretreatments. Biologia plantarum, 36(4), 511-517. doi: 10.1007/BF02921170
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