biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 36:65, 1994 | DOI: 10.1007/BF02921271

Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability

P. Binarová1, M. Cvikrová2, T. Havlický1, J. Eder2, J. Plevková1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic
2 Praha 6, Czech Republic

Glyphosate tolerant cell lines were selected from highly embryogenic cell suspension culture ofMedicago sativa L. Resistant cell lines showed significant reduction of embryogenic ability and during long-term culture in the presence of glyphosate gradual loss of this ability was observed. After glyphosate treatment the increased activity of 5-enolpyruvylshikimate-3-phosphate synthase in tolerant cell lines overcame the block in aromatic amino acid synthesis which was observed in control cell lines. Glyphosate caused marked increase in the content of shikimic acid in both control and tolerant cell lines but the accumulation of shikimic acid was considerably lower in tolerant calli. Significant qualitative and quantitative differences were found in the content of individual phenolic acids. The considerable decrease in the amount of cinnamic acid derivates and broader spectrum of hydroxybenzoic acids suggest in tolerant cell lines the activation of alternative pathway not regulated by phenylalanine ammonia lyase. The possible role of altered pool of phenolic acids on the embryogenic ability is discussed.

Received: February 9, 1993; Accepted: June 1, 1993; Published: January 1, 1994  Show citation

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Binarová, P., Cvikrová, M., Havlický, T., Eder, J., & Plevková, J. (1994). Changes of shikimate pathway in glyphosate tolerant alfalfa cell lines with reduced embryogenic ability. Biologia plantarum, 36(1), 65. doi: 10.1007/BF02921271
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