biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 36:133, 1994 | DOI: 10.1007/BF02921280

Effect of UV-C on peroxidase isoenzymes in axillary bud cultures ofVitis species differing in fungal resistance toPlasmopara viticola

J. M. Zapata1, A. A. Calderón1, M. A. Pedreño1, R. Muñoz1, A. Ros Barceló1
1 Department of Plant Biology (Plant Physiology), University of Murcia, Spain

The effect of shortwave (250 nm) UV radiation (UV-C) on the level of peroxidase activity and peroxidase isoenzyme patterns in leaves of resistant ([Vitis vinifera x Viris riparia] x Vitis rupestris andVitis rupestris) and susceptible (Vitis vinifera) grapevine species toPlasmopara viticola (downy mildew) was studied. The results show that although UV-C did not produce significant changes in peroxidase activity in susceptible species, and only minor changes in resistant species, treatment with UV-light induces an acidic isoperoxidase (isoperoxidase A1), capable of oxidising 4-hydroxystilbenes in resistant species. It was named HSPrx 2. Since peroxidase is apparently the enzyme responsible for ε-viniferin synthesis from resveratrol in grapevines, a close relationship between this peroxidase isoenzyme and ε-viniferin synthesis which occurs in grapevine leaves after UV-C treatment must be expected.

Received: December 9, 1992; Accepted: January 10, 1993; Published: January 1, 1994  Show citation

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Zapata, J.M., Calderón, A.A., Pedreño, M.A., Muñoz, R., & Ros Barceló, A. (1994). Effect of UV-C on peroxidase isoenzymes in axillary bud cultures ofVitis species differing in fungal resistance toPlasmopara viticola. Biologia plantarum, 36(1), 133. doi: 10.1007/BF02921280
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