biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 34:15-22, 1992 | DOI: 10.1007/BF02925784

Somatic embryogenesis and plant regeneration inPisum sativum L.

J. Stejskal1, M. Griga1
1 Oseva Research and Breeding Institute of Technical Crops and Legumes, ©umperk, Czechoslovakia

Somatic embryogenesis was induced in immature zygotic embryos of pea (Pisum sativum L.), synthetic auxins α-naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid (picloram, PIC) being used. Only one (line HM-6) of 46 genotypes tested exhibited good potential for somatic embryogenesis. 2,4-D was found as the best somatic embryo inductor. Three different ways of somatic embryo conversion have been described. Plantlets from individual somatic embryos were micropropagated as somaclones and subsequently rooted. A sterile morphological mutant has been found within a group of fertile plants of T0-generation. Sufficient amount of T1-seeds is available for somaclonal variation studies.

Received: February 26, 1991; Accepted: September 4, 1991; Published: January 1, 1992  Show citation

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Stejskal, J., & Griga, M. (1992). Somatic embryogenesis and plant regeneration inPisum sativum L. Biologia plantarum, 34(1-2), 15-22. doi: 10.1007/BF02925784
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