biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 34:203, 1992 | DOI: 10.1007/BF02925869

Phenolic accumulation and peroxidase activity inin vitro selected alfalfa callus cultures resistant to filtrate ofFusarium spp.

M. Hrubcová1, M. Cvikrová1, L. Meravy1, J. Eder1, P. Binarova2
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6, Czechoslovakia
2 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Olomouc, Czechoslovakia

Changes in the phenylalanine ammonia-lyase (PAL) activity, accumulation of phenolic acids and ionically-bound peroxidase activity in thein vitro selected embryogenic and nonembryogenicMedicago sativa callus cultures resistant to the filtrate ofFusarium spp. were found. The PAL activity in bothin vitro selected cultures during a 4-week cultivation on a medium with phytotoxins was higher than in the control calli grown on a medium without toxin. The filtrate fromFusarium spp. evoked an increase in the contents of all determined phenolic acids in the selected calli. They occurred predominantly bound as esters. The most pronounced portions in the elevated acids level were of ester-bound p-hydroxybenzoic, vanillic, p-coumaric and ferulic acids. The ionic cell wall-bound peroxidase activity in both selected calli cultivated on a medium with a filtrate was twice as high as the activity determined in the control cultures. The activity of soluble peroxidase was not influenced by challenge with a filtrate. No significant differences were found between thein vitro selected embryogenic and nonembryogenic alfalfa callus cultures in the response to the phytotoxic filtrate.

Received: September 6, 1991; Accepted: October 15, 1991; Published: May 1, 1992  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Hrubcová, M., Cvikrová, M., Meravy, L., Eder, J., & Binarova, P. (1992). Phenolic accumulation and peroxidase activity inin vitro selected alfalfa callus cultures resistant to filtrate ofFusarium spp. Biologia plantarum, 34(3-4), 203. doi: 10.1007/BF02925869
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bajaj, K.L., Singh, P.P., Kaur, G.: Effect of cercosporin toxin on polyphenol metabolism in mungbean (Vigna radiata L. Wilczek) leaves. - Biochem. Physiol. Pflanzen180: 621-624, 1985. Go to original source...
    2. Beaudoin-Eagan, L.D., Thorpe, T.A.: Turnover of shikimate pathway metabolites during shoot initiation in tobacco callus cultures. - Plant Cell Physiol.25: 913-921, 1984.
    3. Binarová, P., Novák, J.F.: Studies on embryogenesis into different types of cell cultures ofMedicago sativa. - Acta Univ. Agr. (Brno)23: 293-296, 1985.
    4. Binarová, P., Nedìlník, J., Fellner, M., Nedbálková, B.: Selection for resistance to filtrates ofFusarium spp. in embryogenic cell suspension culture ofMedicago sativa L. - Plant Cell Tissue Organ Cult.22: 191-196, 1990. Go to original source...
    5. Blaydes, D.F.: Interaction of kinetin and various inhibitors of the growth of soybean tissue. - Physiol. Plant.19: 748-753, 1966. Go to original source...
    6. Bolwell, G.P., Robbins, M.O., Dixon, R.A.: Metabolic changes in elicitor-treated bean cells. Enzymic responses associated with rapid changes in cell wall components. - Eur. J. Biochem.148: 571-578, 1985. Go to original source...
    7. Bray, H.C., Thorpe, W.V.: Determination of phenols. - In: Glick, D. (ed.): Methods of Biochemical Analysis. Vol. 1. Pp. 27-52. Academic Press, New York 1954. Go to original source...
    8. Castillo, F.J., Penel, C, Greppin, H.: Peroxidase release induced by ozone inSedum album leaves. -Plant Physiol.74: 846-851, 1984. Go to original source...
    9. Cvikrová, M., Hrubcová, M., Meravý L., Pospí¹il, F.: Changes in the content of phenolic substances during the growth ofNicotiana tabacum cell suspension culture. - Biol. Plant.30: 185-192, 1988. Go to original source...
    10. Dixon, R.A.: The phytoalexin response: Elicitation, signalling and the control of host gene expression. - Biol. Rev.61: 239-291, 1986. Go to original source...
    11. Ebel, J.: Phytoalexin synthesis: The biochemical analysis of the induction process. - Annu. Rev. Phytopathol.24: 235-264, 1986. Go to original source...
    12. Fanner, E.E.: Effects of fungal elicitor on lignin biosynthesis in cell suspension cultures of soybean. - Plant Physiol.78: 338-342, 1985. Go to original source...
    13. Ferraris, L., Abbattista Gentile, I., Matta, A.: Variations of phenols concentration as a consequence of stresses that induce resistance toFusarium wilt of tomato. - J. Plant Protect.94: 624-629, 1987.
    14. Fry, S.C.: Polymer-bound phenol as natural substrate of peroxidases. - In: Greppin, H., Penel, C., Gaspar, T. (ed.): Molecular and Physiological Aspects of Plant Peroxidases. Pp. 169-182. Université de Genève, Genève 1986.
    15. Gaspar, T., Penel, C., Greppin, H.: Peroxidases: Structures and catalytic reactions, biosynthesis, transport and location, physiological roles. - Bull. Group Polyphenols13: 159-176, 1986.
    16. Harkin, J.M., Obst, J.R.: Lignification in trees: indication of exclusive peroxidase participation. - Science180: 296-297, 1973. Go to original source...
    17. Jorrin, J., Dixon, R.A.: Stress responses in alfalfa (Medicago sativa L.): II. Purification, characterization, and induction of phenylalanine ammonia-lyase isoforms from elicitor-treated cell suspension cultures. - Plant Physiol.92: 447-455, 1990. Go to original source...
    18. Kurosaki, F., Tashiro, N., Nishi, A.: Induction of chitinase and phenylalanine ammonia-lyase in cultured carrot cells treated with fungal mycelial walls. - Plant Cell Physiol.27: 1587-1591, 1986.
    19. Latunde-Dada, A.O., Lucas, J.A.: Influence of temperature on host resistance and fungal sensitivity to medicarpin in lucerne callus lines infected withVerticillium albo-atrum. - Physiol. mol. Plant Pathol.28: 89-97, 1986. Go to original source...
    20. Latunde-Dada, A.O., Dixon, R.A., Lucas, J.A.: Induction of phytoalexin biosynthetic enzymes in resistant and susceptible lucerne callus lines infected withVerticillium albo-atrum. - Physiol. mol. Plant Pathol.31: 15-23, 1987. Go to original source...
    21. Legrand, M., Frilig, B., Hirth, L.: Enzymes of the phenylpropanoid pathway and the necrotic reaction of hypersensitive tobacco to tobacco mosaic virus. - Phytochemistry15: 1353-1359, 1976. Go to original source...
    22. Mato, M.C., Rua, M.L., Ferro, E.: Changes in levels of peroxidases and phenolics during root formation inVitis culturedin vitro. - Physiol. Plant.72: 84-88, 1988. Go to original source...
    23. Mayer, A.M.: Polyphenol oxidases in plants-recent progress. - Phytochemistry26: 11-20, 1987. Go to original source...
    24. Mayer, A.M., Harel, E.: Polyphenol oxidase in plants. - Phytochemistry18: 193-215, 1979. Go to original source...
    25. Meravy, L.: The effect of calcium deficiency on the activity of ammonia-lyases in the shikimic pathway. - Biol. Plant.21: 427-433, 1979. Go to original source...
    26. Nedìlník, J.: The production of phytotoxic metabolies ofFusarium oxysporum andFusarium solani in liquid media.- Ochr. Rost. (Praha)25: 261-269, 1989. [In Czech.]
    27. Niemann, G.J., Baayen, R.P.: Phenol metabolism and resistance againstFusarium oxysporum f.sp. dianthi inDianthus carophyllus. - Bull. Liaison Groupe Polyphenols14: 185-188, 1988.
    28. Pascholati, S.F., Heim, D., Nicholson, R.L.: Phenylalanine ammonia-lyase and susceptibility of the maize mesocotyl toHelminthosporium maydis. - Physiol. Plant Pathol.27: 345-356, 1985. Go to original source...
    29. Van Loon, L.C., Geelem, J.L.M.C.: The relation of polyphenoloxidase and peroxidase to symptom expression in tobacco var. "Samsun NN" after infection with tobacco mosaic virus. - Acta phytopathol. Acad. Sci. hung.6: 9-20, 1971.
    30. Van Loon, L.C.: The significance of changes in peroxidase in diseased plants. - In: Greppin, H., Penel, C., Gasper, T. (ed.): Molecular and Physiological Aspects of Plant Peroxidases. Pp. 405-418. Université de Genéve, Genéve 1986.
    31. Zádor, E., Köves, E., Szabo, M.: Phenolic materials in auxin heterotroph and autotroph (habituated) tobacco callus tissues. - Biochem. Physiol. Pflanzen180: 125-131, 1985. Go to original source...

    Return to the content