biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 34:335, 1992 | DOI: 10.1007/BF02925895

Nuclear DNA characterization of two species ofVicia:Vicia bithynica L. andVicia narbonensis L.

M. Frediani1, O. Sassoli2, R. Cremonini1
1 Dipartimento di Scienze Botaniche delta Universita, Pisa, Italy
2 Dipartimento di Biologia delle Piante Agrarie della Universita, Pisa, Italy

The speciesVicia bithynica andVicia narbonensis, from the same subgeneric section ofVicia faba, show variations in nuclear DNA content Nuclear DNAs, extracted from root tips of the twoVicia species, were characterized by thermal denaturation, analytical ultracentrifugation and reassociation kinetics. The thermal denaturations of DNA, the number of DNA components reassociating with second order kinetics, the proportion of repeated DNA sequences, the frequency of the repeated DNA classes are reported and compared to previous data onVicia faba DNA. Feulgen absorptions at different thresholds of optical density+ of interphase nuclei in cytological preparations of the root meristems ofV. bithynica andV. narbonensis are determined and compared withV. faba analogous determinations. The results, confirming that plant genome is highly flexible, are discussed in relation to other data on the interspecific variations of the nuclear DNA content.

Received: December 11, 1990; Accepted: April 9, 1991; Published: May 1, 1992  Show citation

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Frediani, M., Sassoli, O., & Cremonini, R. (1992). Nuclear DNA characterization of two species ofVicia:Vicia bithynica L. andVicia narbonensis L. Biologia plantarum, 34(3-4), 335. doi: 10.1007/BF02925895
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