biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 35:169-177, 1993 | DOI: 10.1007/BF02925934

Interspecific hybridization inBrassica: Application of flow cytometry for analysis of ploidy and genome composition in hybrid plants

P. S. Sabharwal1, J. Doležel1
1 Department of Genetics and Plant Breeding, University of Agriculture, Prague 6, Czech Republic

Interspecific hybrids from the crosses betweenBrassica campestris, B. carinata, B. juncea andB. napus were obtained throughin vitro ovary and ovule culture. F1 hybrids were studied morphologically and flow cytometry was used to estimate 2C nuclear DNA content both in parentalBrassica species and their hybrids. It was found that in comparison with the A genome, the B and the C genomes ofBrassica contained 26.9 % and 43.9 % more DNA, respectively. This finding may be used to distinguish interspecific hybrids containing various genome combinations. It was concluded that flow cytometric analysis of nuclear DNA content might be useful tool inBrassica breeding.

Received: June 18, 1992; Accepted: February 1, 1993; Published: June 1, 1993  Show citation

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Sabharwal, P.S., & Doležel, J. (1993). Interspecific hybridization inBrassica: Application of flow cytometry for analysis of ploidy and genome composition in hybrid plants. Biologia plantarum, 35(2), 169-177. doi: 10.1007/BF02925934
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