biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 35:191, 1993 | DOI: 10.1007/BF02925938

High level of endogenous cytokinins in transgenic potato plantlets limits photosynthesis

J. Čatský1, J. Pospíšilová1, I. Macháčková1, H. Synková1, N. Wilhelmová1, Z. Šesták1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic

Introduction of the gene for cytokinin synthesis into potato genome lead to a manifold increase in the level of cytokinins (zeatin, zeatin riboside, isbpentenyl-adenine, isopentenyladenosine) in plantlets grownin vitro.The increasing cytokinin level was associated with increasing tendency to teratoma formation, to decreasing leaf net photosynthetic rate and to increasing dark and light respiration rates and CO2 compensation concentration. During plantlet (or teratoma) ontogeny, net photosynthetic rate increased simultaneously with the decrease in cytokinin level. High level of endogenous cytokinins was associated also with lower photochemical activities of both photosystems in isolated chloroplasts.

Received: June 4, 1992; Accepted: March 23, 1993; Published: June 1, 1993  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Čatský, J., Pospíšilová, J., Macháčková, I., Synková, H., Wilhelmová, N., & Šesták, Z. (1993). High level of endogenous cytokinins in transgenic potato plantlets limits photosynthesis. Biologia plantarum, 35(2), 191. doi: 10.1007/BF02925938
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abddelghani, M.O., Suty, L., Chen, J.N., Renaudin, J.P., Teyssendierde la Serve, B.: Cytokinins modulate the steady-state levels of light-dependent and light-independent proteins and mRNA in tobacco cell suspensions. Plant Sci.77: 29-40, 1991. Go to original source...
    2. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris. Plant Physiol.24: 1-15, 1949. Go to original source...
    3. Čatský, J., Tichá, I.: A closed system for measurement of photosynthesis, photorespiration and transpiration rates. Biol. Plant.17: 405-410, 1975. Go to original source...
    4. Chory, J., Aguilar, N., Peto, C.A.: The phenotype ofArabidopsis thaliana det1 mutants suggests a role for cytokinins in greening. In: Jenkins, G.I., Schuch, W. (ed.) : Molecular Biology of Plant Development. Pp. 21-29. Company of Biologists, Cambridge 1991. Go to original source...
    5. Dominov, J.A., Stenzler, L., Lee, S., Schwarz, J.J., Leisner, S., Howell, S.H.: Cytokinins and auxins control the expression of a gene inNicotiana plumbaginifolia cells by feedback regulations. Plant Cell4: 451-461, 1992. Go to original source...
    6. Flores, S., Tobin, E.M.: Benzyladenine modulation of the expression of two genes for nuclear-encoded chloroplast proteins inLemna gibba: Apparent post-transcriptional regulation. Planta168: 340-349, 1986. Go to original source...
    7. Guéra, A., Sabater, B.: Synthesis of chloroplast proteins by barley leaf segments: Effects of senescence induction and kinetin treatment. Acta bot. neerl.41 : 43-49, 1992. Go to original source...
    8. Kaše, M, Čatský,J: Calculator-assisted measurements of photosynthetic, respiration and photorespiration rates in a closed gas exchange system. Biol. Plant.25: 139-146, 1983. Go to original source...
    9. Legocka, A.: Kinetin-induced changes in the synthesis of nucleic acids and proteins in cucumber cotyledons. Acta Physiol. Plant.11: 19-29, 1989.
    10. Macháčková, I., Krekule, J., Eder, J., Seidlová, F., Stmad, M : Cytokinins in photoperiodic induction of flowering inChenopodium species. - Physiol. Plant. in press, 1993. Ondřej, M., Bavrina, T.V., Dudko, N., Hrouda, M., Krekule, J., Lozhnikova, V.N., Macháčková, I. Go to original source...
    11. Seidlová, F., Vlasák, J.: Transgenic tobacco plants with T-DNA phytochrome synthesis genes. Biol. Plant.32:40-48, 1991. Go to original source...
    12. Ondřej, M., Hrouda, M., Karavajko, N.N., Matoušek, J., Mikulovič, T.P., Pavingerová, D., Vlasák, J.: Transformation ofAgrobacterium vectors and the study of functions of plant hormones. In: Krekule, J., Seidlová, F. (ed.) : Signals in Plant Development. Pp. 73 -89. SPB Academic Publishing, The Hague 1989.
    13. Ondřej, M., Macháčková, I., Čatský, J., Eder, J., Hrouda, M., Pospíšilová, J., Synková, H.: Potato transformation by T-DNA cytokinin synthesis gene. Biol. Plant.32: 401 - 406, 1990. Go to original source...
    14. Ooms, G.: Controlling differentiation and endogenous growth regulator content by means of genetic transformations. In: Advances in the Chemical Manipulation of Plant Tissue Cultures. (Monograph No. 16.) Pp. 1 - 17. British Plant Growth Regulator Group, Bristol 1987.
    15. Ooms, G., Lenton, J.R.: T-DNA genes to study plant development: precocious tuberization and enhanced cytokinins inA. tumefaciens transformed potato. Plant mol. Biol.5 : 205-212, 1985. Go to original source...
    16. Pedhadiya, M.D., Vaishnav, P.P., Singh, Y.D.: Development of photosynthetic electron transport reactions under the influence of phytohormones and nitrate nutrition in greening cucumber cotyledons. Photosynth. Res.13: 159-165, 1987. Go to original source...
    17. Robinson, S.P.: Lack of ATP requirement for light stimulation of glycerate transport into intact isolated chloroplasts. Plant Physiol.75: 425-430, 1984. Go to original source...
    18. Schmitt, J.M., Piepenbrock, M.: Regulation of phosphoeno/pyruvate carboxylase and crassulacean acid metabolism inMesembryanthemum crystallinum L. by cytokinin. Modulation of leaf gene expression by root? Plant Physiol.99: 1664-1669, 1992. Go to original source...
    19. Šesták, Z., Čatský, J. (ed.): Photosynthesis Bibliography. Vol. 1-19. W. Junk, The Hague, Dordrecht - Boston - London; SPB Academic Publishing, The Hague 1974-1991. Go to original source...
    20. Šiffel, P., Šindelková, E., Durchan, M., Zajícová, M. : Photosynthetic characteristics ofSolatium tuberosum L. plants transformed byAgrobacterium strains. 1. Pigment apparatus. - Photosynthetica27 : in press, 1992.
    21. Strnad, M., Vaněk, T., Binarová, P., Kamínek, M., Harmš, J.: Enzyme immunoassays for cytokinins and their use for immunodetection of cytokinins in alfalfa cell culture. In: Kutáček, M., Elliott, M.C., Macháčková, I. (ed.) : Molecular Aspects of Hormonal Regulation of Plant Development. Pp. 41-54. SPB Academic Publishing, The Hague 1990.
    22. Teyssendierde laServe, B., Angelos, M., Péaud-Lenoël, C: Cytokinins modulate the expression of genes encoding the protein of the light-harvesting chlorophylla/b complexes. Plant mol. Biol.5: 155-163, 1985. Go to original source...
    23. Vaňková, R., Hsiao, K.-C, Bornman, C.H., Gaudinová, A.: Effect of synthetic cytokinins on levels of endogenous cytokinins and respiration patterns ofBeta vulgaris cells in suspension. J. Plant Growth Regul.10: 197-199, 1991. Go to original source...

    Return to the content