biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 35:609, 1993 | DOI: 10.1007/BF02928039

Soluble pathogenesis-related proteins ofCapsicum annuum leaves induced by cucumber mosaic virus infection

M. E. Candela1, A. Espín1, M. D. Alcázar1
1 Department of Plant Biology, Faculty of Biology, University of Murcia, Murcia, Spain

Eight major pathogenesis related (PR) proteins were found in a soluble extract of cucumber mosaic virus (CMV) infectedCapsicum annuum leaves. None of them was present in the soluble extract of sham-inoculated controls. The proteins were detected by two-dimensional polyacrylamide gel electrophoresis using (2D-PAGE)PhastSystem, native-PAGE in the first dimension and SDS-PAGE in the second. Two major acidic PR-proteins were identified on the basis of their relative molecular mass (Mr), PR1 of 15.3 kD and PR2 of 29 kD. Six proteins were basic and were identified as PRla of 15.9 kD, PRlb of 15.0 kD, PRlc of 15.9 kD, PR2 of 27.0 kD, PR3 of 36.3 kD and PR4 of 48.8 kD.

Received: June 26, 1992; Accepted: November 4, 1992; Published: December 1, 1993  Show citation

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Candela, M.E., Espín, A., & Alcázar, M.D. (1993). Soluble pathogenesis-related proteins ofCapsicum annuum leaves induced by cucumber mosaic virus infection. Biologia plantarum, 35(4), 609. doi: 10.1007/BF02928039
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