biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 35:329-339, 1993 | DOI: 10.1007/BF02928506

Effect of anti-microtubular drug amiprophos-methyl on somatic embryogenesis and DNA ploidy levels in alfalfa and carrot cell suspension cultures

P. Binarová1, J. Doležel1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

A short treatment with the anti-microtubular drug amiprophos-methyl (APM) blocked somatic embryogenesis in alfalfa(Medicago sativa L.) and carrot (Daucus carota L.). The interruption was temporary and restoration of somatic embryogenesis was observed in long-term cultures. In addition to the effect on somatic embryogenesis, APM treatment induced polyploidization the extent of which was concentration dependent. In long-term alfalfa cultures, APM-induced loss of somatic embryogenesis led to ploidy instability and to a shift to DNA aneuploidy. Critical stages of somatic embryogenesis sensitive to disruption of microtubule-mediated processes were determined in carrot cell cultures. Complete embryogenic arrest occurred when APM was added within the first 5 d of embryogenesis from single cells. The role of the cytoskeleton in the first events of somatic embryogenesis and the relation between totipotency and ploidy stabilityin vitro is discussed.

Received: September 30, 1991; Accepted: February 16, 1993; Published: September 1, 1993  Show citation

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Binarová, P., & Doležel, J. (1993). Effect of anti-microtubular drug amiprophos-methyl on somatic embryogenesis and DNA ploidy levels in alfalfa and carrot cell suspension cultures. Biologia plantarum, 35(3), 329-339. doi: 10.1007/BF02928506
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