biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 35:393, 1993 | DOI: 10.1007/BF02928516

Photosynthesis and water relations in transgenic tobacco plants with T-DNA carrying gene 4 for cytokinin synthesis

J. Čatský1, J. Pospíšilová1, I. Macháčková1, N. Wilhelmová1, Z. Šesták1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic

Expression of the gene 4 for cytokinin synthesis in two clones of transgenic tobacco plants resulted in slightly higher(ca. 10 - 20 %) levels of endogenous cytokinins -zeatin, zeatin riboside and isopentenyladenosine. This was associated with changes in photosynthetic processes on chloroplast and leaf level. In comparison with nontransformed plantlets a higher degree of grana stacking was found in both clones of transgenic plants which indicated a higher proportion of photosystem 2 lightharvesting complex in thylakoids. This was supported by a decreased chlorophylla/b ratio in the separated grana fraction. The rate of leaf net photosynthetic rate (measured as CO2 uptake) wasca. 25 % higher in both clones than in nontransformed plants. Also the rates of dark and light respiration, and CO2 compensation concentration were higher in transgenic plants. On the other hand, parameters of water relations (water, osmotic and pressure potentials, stomatal and cuticular transpiration rates) were not significantly affected in transgenic tobacco plants.

Received: December 20, 1992; Accepted: April 16, 1993; Published: September 1, 1993  Show citation

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Čatský, J., Pospíšilová, J., Macháčková, I., Wilhelmová, N., & Šesták, Z. (1993). Photosynthesis and water relations in transgenic tobacco plants with T-DNA carrying gene 4 for cytokinin synthesis. Biologia plantarum, 35(3), 393. doi: 10.1007/BF02928516
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