biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:439-442, 2005 | DOI: 10.1007/s10535-005-0024-7

Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants

S. Echeverrigaray1,*, R. Basso1, L. B. Andrade1
1 Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil

Axillary buds from adult field-grown plants of Lavandula dentata L. were used to evaluate the effect of growth regulators and culture media on the in vitro shoot proliferation and growth. The highest multiplication rate was obtained using Murashige and Skoog (MS) medium supplemented with a combination of 2.2 μM of benzyladenine and 2.5 μM indole-3-butyric acid. The best condition for rooting was MS medium plus 2.5 μM naphthaleneacetic acid. Rooted plantlets were successfully transferred to soil. Short-term culture derived plants (6 month) exhibited a normal development, but a low frequency of not heritable morphological changes were detected in long term culture derived plants (more than 1 year).

Keywords: lavender; in vitro propagation; bud breaking
Subjects: auxins; bud; cytokinins; in vitro culture, axillary buds proliferation, bud breaking and regeneration; in vitro culture, micropropagation; Lavandula dentata; lavender, micropropagation from axillary buds; medicinal plants; nutrient medium LS (Linsmaier and Skoog); nutrient medium MS (Murashige and Skoog); nutrient medium NN (Nitch and Nitch); nutrient medium QL (Quoirin and Lepoivre)

Received: March 10, 2004; Accepted: January 18, 2005; Published: September 1, 2005  Show citation

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Echeverrigaray, S., Basso, R., & Andrade, L.B. (2005). Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants. Biologia plantarum, 49(3), 439-442. doi: 10.1007/s10535-005-0024-7
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