biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:93-97, 2007 | DOI: 10.1007/s10535-007-0019-7

Effect of seed soaking with thiols on the antioxidant enzymes and photosystem activities in wheat subjected to water stress

N. S. Nathawat1, J. S. Nair2, S. M. Kumawat1, N. S. Yadava1, G. Singh1, N. K. Ramaswamy2,*, M. P. Sahu1, S. F. D'Souza2
1 Rajasthan Agricultural University, Bikaner, India
2 Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India

Photosystem 1 and 2 and antioxidant enzyme activities were determined in wheat (Triticum aestivum L. cv. Sonalika) leaves. Seedlings from both control seeds and seeds soaked in solutions like dithiothreitol, thioglycollic acid and thiourea were subjected to water stress induced by polyethylene glycol. Photosystem 1 and 2 activities were less inhibited by water stress due to seed soaking with sulphydryl compounds. The changes in activities of antioxidant enzymes induced by water stress were higher in seedlings from thiol-pretreated seeds than from water-soaked seeds.

Keywords: oxygen evolution; peroxidase; photosynthesis; polyethylene glycol; seed pretreatment; sulphydryl compounds
Subjects: antioxidants, antioxidant enzymes; ascorbate peroxidase; catalase; chlorophyll a,b; chloroplast isolation; dithiothreitol; glutathione reductase; glutathione-S-transferase; guaiacol peroxidase; oxygen evolution; peroxidase; photosystems 1,2; random amplified polymorphic DNA (RAPD); reactive oxygen species (ROS); superoxide dismutase (SOD); thiourea; Triticum aestivum; water stress; wheat

Received: March 18, 2005; Accepted: December 20, 2005; Published: March 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Nathawat, N.S., Nair, J.S., Kumawat, S.M., Yadava, N.S., Singh, G., Ramaswamy, N.K., Sahu, M.P., & D'Souza, S.F. (2007). Effect of seed soaking with thiols on the antioxidant enzymes and photosystem activities in wheat subjected to water stress. Biologia plantarum, 51(1), 93-97. doi: 10.1007/s10535-007-0019-7
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Asada, K.: The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons.-Annu. Rev. Plant Physiol. mol. Biol. 50: 601-639, 1999. Go to original source...
    3. Becana, M., Aparicio-Tejo, P., Irigoyen, J.J., Sanchez-Diaz, M.: Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa.-Plant Physiol. 82: 1169-1171, 1986. Go to original source...
    4. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Burman, U., Garg, B.K., Kathju, S.: Interactive effects of thiourea and phosphorus on clusterbean under water stress.-Biol. Plant. 48: 61-65, 2004. Go to original source...
    6. Chance, B., Maehly, A.C.: Assay of catalase and peroxidases.-Methods Enzymol. 2: 764-755, 1955. Go to original source...
    7. De Agazio, M., Zacchini, M.: Dimethylthiourea, a hydrogen peroxide trap, partially prevents stress effects and ascorbate peroxidase increase in spermidine-treated maize roots.-Plant Cell Environ. 24: 237-244, 2001. Go to original source...
    8. Deneke, S.M.: Thiol-based antioxidants.-Curr. Topics Cell Regul. 36: 151-180, 2000. Go to original source...
    9. Giannopolitis, C.N., Ries, S.K: Superoxide dismutase. I. Occurrence in higher plants.-Plant Physiol. 59: 309-314, 1977. Go to original source...
    10. Hoagland, D.R., Arnon, D.I.: The water culture method for growing plants without soil.-California agr. Experimental Station Circular 347: 1-39, 1938.
    11. Horling, F., Lamkemeyer, P., Konnig, J., Finkemeir, L., Kandlbinder, A., Baier, M., Dietz, K.-J.: Divergent light-, ascorbate-, and oxidative stress-dependent regulation expression of the peroxiredoxin gene family in Arabidopsis.-Plant Physiol. 131, 317-325, 2003. Go to original source...
    12. Izawa, S.: Acceptors and donors for chloroplast electron transport.-Methods Enzymol. 69: 413-434, 1980. Go to original source...
    13. Izawa, S., Good, N.E.: The stoichiometric relation of phosphorylation to electron transport in isolated chloroplast.-Biochim. biophys. Acta 162: 380-391, 1968. Go to original source...
    14. Malanga, G., Kozak, R.G., Puntarulo, S.: N-acetyl cysteine-dependent protection against UV-B damage in two photosynthetic organisms.-Plant Sci. 141: 129-137, 1999. Go to original source...
    15. Mannervik, B., Guthenberg, C.: Glutathione transferase (human placenta).-Methods Enzymol. 77: 231-235, 1981. Go to original source...
    16. Meister, A., Anderson, M.E.: Glutathione.-Annu. Rev. Biochem. 52: 711-760, 1983. Go to original source...
    17. Misra, A.N., Biswal, U.C.: Effect of phytohormone on chlorophyll degradation of chloroplast in vivo and in vitro.-Protoplasma 105: 1-8, 1980. Go to original source...
    18. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast.-Plant Cell Physiol. 22: 867-880, 1981.
    19. Nayak, L., Biswal, B., Ramaswamy, N.K., Iyer, R.K., Nair, J.S., Biswal, U.C.: Ultraviolet-A induced changes in photosystem II of thylakoids: effects of senescence and high growth temperature.-J. Photochem. Photobiol. B 70: 59-65, 2003. Go to original source...
    20. Noctor, G., Foyer, C.H.: Ascorbate and glutathione: keeping active oxygen under control.-Annu. Rev. Plant Physiol. mol. Biol. 49: 249-279, 1998. Go to original source...
    21. Sahu, M.P., Singh, D.: Role of thiourea in improving productivity of wheat (Triticum aestivum L.).-Plant Growth Regul. 14: 169-173, 1995. Go to original source...
    22. Sahu, M.P., Solanki, N.S.: Role of sulphydryl compounds in improving dry matter partitioning and grain production of maize (Zea mays L.).-J. Agron. Crop Sci. 167: 356-359, 1991. Go to original source...
    23. Sahu, M.P., Solanki, N.S., Dashora, L.N.: Effects of thiourea, thiamine and ascorbic acid on growth and yield of maize (Zea mays L.).-J. Agron. Crop Sci. 171: 65-69, 1993. Go to original source...
    24. Sen, C.K.: Cellular thiols and redox-regulated signal transduction.-Curr. Topics cell. Regul. 36: 1-30, 2000. Go to original source...
    25. Shaedle, M., Bassham, J.A.: Chloroplast glutathione reductase.-Plant Physiol. 59: 1011-1012, 1977. Go to original source...
    26. Werdan, K., Heldt, H.W., Milovancev, M.: The role of pH in the regulation of carbon fixation in the chloroplast stroma: studies on CO2 fixation in the light and dark.-Biochim. biophys. Acta 396: 276-282, 1975. Go to original source...
    27. Zhu, B-Z., Antholine, W.E., Frei, B.: Thiourea protects against copper-induced oxidative damage by formation of a redoxinactive thiourea-copper complex.-Free Radical. Biol. Medicine 32: 1333-1338, 2002. Go to original source...

    Return to the content