biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:325-328, 2009 | DOI: 10.1007/s10535-009-0059-2

In vitro direct organogenesis and regeneration of Medicago sativa

J. J. Li1, Y.M. Wu1,*, T. Wang1, J. X. Liu1
1 College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China

A rapid and efficient plant regeneration protocol for a wide range of alfalfa genotypes was developed via direct organogenesis. Through a successive excision of the newly developed apical and axillary shoots, a lot of adventitious buds were directly induced from the cotyledonary nodes when hypocotyl of explants were vertically inserted into modified Murashige and Skoog (MS) medium supplemented with 0.025 mg dm-3 thidiazuron (TDZ) and 3 mg dm-3 AgNO3. When the lower part of shoots excised from explants were immersed into the liquid medium with 1.0 mg dm-3 α-naphthaleneacetic acid (NAA) for 2 min, and then transferred to hormone free half-strength MS medium, over 83.3 % of the shoots developed roots, and all plantlets could acclimatize and establish in soil. The protocol has been successfully applied to eight genotypes, with regeneration frequencies ranging from 63.8 to 82.5 %.

Keywords: alfalfa; cotyledonary node; mature embryo; silver nitrate; thidiazuron; vitrification
Subjects: alfalfa; auxins; cytokinins; ex vitro transfer; in vitro culture, direct regeneration; silver nitrate

Received: May 10, 2007; Accepted: March 15, 2008; Published: June 1, 2009  Show citation

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Li, J.J., Wu, Y.M., Wang, T., & Liu, J.X. (2009). In vitro direct organogenesis and regeneration of Medicago sativa. Biologia plantarum, 53(2), 325-328. doi: 10.1007/s10535-009-0059-2
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