biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:797-799, 2009 | DOI: 10.1007/s10535-009-0147-3

The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron

D. Pavingerová1,*
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

The influence of cytokinin thidiazuron (TDZ) and auxin indole-3-acetic acid (IAA) on in vitro shoot organogenesis of fifteen Rhododendron genotypes was investigated and a protocol for high frequency adventitious shoot regeneration from leaf explants was developed. High genotypic variation was observed and regeneration frequencies ranged from 0 to 100 %. Genotype Ovation had the highest number of shoots (26.4 per explant) after 12 weeks on medium with 0.57 µM IAA and 1.20 µM TDZ, but only 65 % of explants regenerated. Catawbiense Grandiflorum had 17.7 shoots per explant and 75 % regeneration on medium with 5.70 µM IAA and 0.45 µM TDZ and Van Werden Poelman had 14.3 shoots per explant and 100 % regeneration on medium with 0 57 µM IAA and 0.45 µM TDZ.

Keywords: auxin; cytokinin; plant growth regulators; tissue culture
Subjects: auxins; in vitro culture, direct regeneration; Rhododendron; thidiazuron

Received: January 26, 2009; Accepted: August 11, 2009; Published: December 1, 2009  Show citation

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Pavingerová, D. (2009). The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron. Biologia plantarum, 53(4), 797-799. doi: 10.1007/s10535-009-0147-3
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