biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:131-138, 2014 | DOI: 10.1007/s10535-013-0358-5

Physiological and ultra-structural changes in Brassica napus seedlings induced by cadmium stress

B. Ali1, P. Qian1, R. Jin2, S. Ali4, M. Khan1, R. Aziz3, T. Tian1, W. Zhou1,2,*
1 Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, China
2 Agricultural Experiment Station, Zhejiang University, Hangzhou, China
3 College of Environment and Resource Science, Zhejiang University, Hangzhou, China
4 Department of Environmental Sciences, Government College University, Faisalabad, Pakistan

The effects of cadmium on physiological and ultrastructural characteristics were evaluated in 6-d-old seedlings of two Brassica napus L. cultivars Zheda 619 and ZS 758. Results show that Cd at lower concentration (100 μM) stimulated the seedling growth but at higher concentration (500 μM) inhibited the growth of both cultivars, decreased content of photosynthetic pigments, activities of antioxidant enzymes, and increased the content of malondialdehyde and reactive oxygen species. Cd content in different parts of seedlings was higher in ZS 758 than in Zheda 619. Electron micrographs illustrated that 500 μM Cd severely damaged the leaf and root tip cells of both cultivars. Under Cd stress, the size and number of starch grains, plastoglobuli, and lipid bodies in the chloroplasts increased. In the root tip cells, enlarged vacuoles, diffused cell walls, and undeveloped mitochondria were detected.

Keywords: antioxidant enzymes; chlorophyll; chloroplasts; mitochondria; oilseed rape; reactive oxygen species
Subjects: cadmium; reactive oxygen specied; growth; chlorophyll content; carotenoids; proteins; ascorbate peroxidase; catalase; peroxidase; glutathione reductase; superoxide dismutase; malondialdehyde; hydrogen peroxide; superoxide anion; ultrastructure; chloroplast; mitochondria; oilseed rape

Received: November 29, 2012; Revised: May 7, 2013; Accepted: May 9, 2013; Published: March 1, 2014  Show citation

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Ali, B., Qian, P., Jin, R., Ali, S., Khan, M., Aziz, R., Tian, T., & Zhou, W. (2014). Physiological and ultra-structural changes in Brassica napus seedlings induced by cadmium stress. Biologia plantarum, 58(1), 131-138. doi: 10.1007/s10535-013-0358-5
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