biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:1-11, 1997 | DOI: 10.1023/A:1000342818872

Growth and nocturnal acid accumulation during early ontogeny of Agave attenuata grown in nutrient solution and in vitro culture

H. Wen1, J. Wagner1, W. Larcher1
1 Institute of Botany, Innsbruck, Austria

Dry matter production of shoots and roots and the diurnal fluctuation of titratable acidity of single leaves were investigated in the CAM plant Agave attenuata during the first 70 d after germination. The plants were grown either in vermiculite sub-irrigated with a nutrient solution or in in vitro cultures on an inorganic nutrient agar. Two types of culture tube covers were used: either airtight closures or polypropylene caps with membranes permeable to air.
In the earliest ontogenetic phases of development (cotyledon and primary leaf stage), the plants were already able to carry out considerable nocturnal organic acid accumulation. In vitro cultivated plants, from the beginning of their development, were also capable of diurnal acid fluctuation, though of distinctly weaker activity than the pot plants. The mean relative growth rates (RGR) of pot culture plantlets approached a third of perennial herbaceous plants. Plantlets grown in in vitro culture reached only half to the one quarter of the RGR of pot plants. The reduced yield could be attributed to the low CO2 supply in the culture tubes and the less than optimal water and nutrient supply in the agar medium.

Keywords: Crassulacean acid metabolism; dry mass; nocturnal acid accumulation; relative growth rate

Prepublished online: January 1, 1997; Published: July 1, 1997  Show citation

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Wen, H., Wagner, J., & Larcher, W. (1997). Growth and nocturnal acid accumulation during early ontogeny of Agave attenuata grown in nutrient solution and in vitro culture. Biologia plantarum, 39(1), 1-11. doi: 10.1023/A:1000342818872
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