Biologia plantarum 39:91-97, 1997 | DOI: 10.1023/A:1000365224324
Biochemical aspects of almond microcuttings related to in vitro rooting ability
- 1 Istituto Sperimentale per la Frutticoltura, Rome, Italy
- 2 Institut de Botanique, Université de Liège, Liège, Belgique
Microcuttings of seven genotypes of almond (Prunus dulcis Mill) used as peach rootstocks, showed different rooting ability in vitro. No direct relationship was found between peroxidase activity and total phenol content, determined in the whole microcuttings prior to the root inducing treatment, and the rooting ability of these genotypes. However, a positive relationship was found between free indole-3-acetic acid (IAA) level and IAA-aspartate and the rooting response. After transferring into the rooting medium, peroxidase activity of the easiest-to-root genotype increase up to a peak and then declined and the total phenol content showed an opposite trend. These variations did not occur in the most difficult-to-root genotype.
Keywords: endogenous IAA; IAA-aspartate; peroxidase; phenols; Prunus dulcis; rhizogenesis
Prepublished online: January 1, 1997; Published: July 1, 1997 Show citation
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References
- Alvarcz, R., Nissen, S.J., Sutter, E.G.: Relationship between indole-3-acetic acid levels in apple (Malus pumila Mill) rootstocks cultured in vitro and adventitious root formation in the presence of indole-3-butyric acid.-Plant Physiol. 89: 439-443, 1989.
Go to original source... - Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976.
Go to original source... - Bray, M., Thorpe, G.: Analysis of phenolic compounds of interest in metabolism.-In: Glick, D. (ed.): Methods of Biochemical Analysis. Pp. 27-57. Interscience Publication, New York 1954.
Go to original source... - Caboni, E.: Peroxidase activity and in vitro rooting in an almond (Prunus dulcis Mill) genotype.-Acta Hort. 373: 201-205, 1994.
Go to original source... - Caboni, E., Damiano, C.: Rooting in two almond genotypes.-Plant Sci. 96: 163-165, 1994.
Go to original source... - Davis, T.D., Haissig, B.E., Sankhla, N.: Adventitious Root Formation in Cuttings.-Dioscorides Press, Portland 1988.
- Foret, Y., Maldiney, R., Sotta, B., Miginiac, E.: Rhizogenesis and auxin and abscisic acid levels in free clones of different aged Sequoia sempervirens (Endl.) trees.-Compt. rend. Acad. Sci. Paris 303: 135-138, 1986.
- Gaspar, T., Kevers, C., Hausman, J.F., Berthon, J.Y., Ripetti, V.: Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots-Agronomic 12: 757-765, 1992.
Go to original source... - Haissig, B.E.: Metabolic process in adventitious rooting of cuttings.-In: Jackson, M.B. (ed.): New Root Formation in Plants and Cuttings. Pp. 191-222. Martinus Nijhoff Publ., Dordrecht 1986.
Go to original source... - Jones, O.P., Hatfield, G.S.: Root initiation in apple shoot cultured in vitro with auxin and phenolic compounds.-J. hort. Sci. 51: 495-499, 1976.
Go to original source... - Le, C.L.: Influence of temperature on in vitro root initiation and development of apple rootstock M26.-HortScience 20: 451-452, 1985.
Go to original source... - Legrand, B.: Action de la lumière sur les peroxydases et sur la teneur en composés phénoliques de tissus de feuilles de Cichorium intybus L. cultivés in vitro.-Biol. Plant. 19: 27-33, 1977.
Go to original source... - Mato, M.C., Vicitez, A.N.: Changes in auxin protectors and IAA oxidases during the rooting of chestnut shoots in vitro.-Physiol. Plant. 66: 491-494, 1986.
Go to original source... - Moncousin, C.: Peroxidase as markers for rooting improvement of clones of Vitis cultured in vitro.-In: Greppin, H., Penel, C., Gaspar, T. (ed.): Molecular and Physiological Aspects of Plant Peroxidases. Pp. 379-385. Univ. Geneva, Geneva 1986.
- Moncousin, C., Gaspar, T.: Peroxidase as marker for rooting improvement of Cynara scolymus L. cultured in vitro.-Biochem. Physiol. Pflanz. 178: 263-271, 1983.
Go to original source... - Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 437-479, 1962.
Go to original source... - Nordstrom, A.C., Eliasson, L.: Levels of endogenous indole-3-acetic acid and indole-3-acetylaspartic acid during adventitious root formation in pea cuttings.-Physiol. Plant. 82: 599-605, 1991.
Go to original source... - Quoirin, G., Boxus, P., Gaspar, T.: Root initiation and isoperoxidases of stem tip cuttings from mature Prunus plants.-Physiol. vég. 12: 165-174, 1974.
- Rugini, E., Verma, D.C.: Micropropagation of difficult-to-propagate almond (Prunus amygdalus Batsch) cultivars.-Plant. Sci. Lett. 28: 273-281, 1983.
Go to original source... - Weigel, U., Horn, W, Hock, B.: Endogenous auxin levels in terminal stem cuttings of Chrysanthemum morifolium during adventitious rooting.-Physiol. Plant. 61: 422-428, 1984.
Go to original source... - Zimmerman, R., Broome, O.C.: Phloroglucinol and in vitro rooting of apple cultivar.-J. amer. Soc. hort. Sci. 106: 648-652, 1981.
Go to original source...



