biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:345-350, 1997 | DOI: 10.1023/A:1001005711955

Effects of ferulic acid on L-malate oxidation in isolated soybean mitochondria

M.A. Sert1, M.L.L. Ferraresi1, Y.R. Bernadelli1, A.M. Kelmer-Bracht1, A. Bracht1, E.L. Ishii-Iwamoto1
1 Department of Biochemistry, University of Maringá, Av. Colombo 5790, Maringá, Brazil

The effects of ferulic acid on L-malate oxidation in mitochondria isolated from soybean (Glycine max L.) seedlings were investigated. Oxygen uptake and the products of L-malate oxidation were measured under two conditions: pH 6.8 and 7.8. At acidic pH, the activity of the NAD+-linked malic enzyme (L-malate:NAD+oxidoreductase [decarboxylating] EC 1.1.1.39) was favoured, whereas at alkaline pH a predominance of the L-malate dehydrogenase activity (L-malate:NAD+oxidoreductase EC 1.1.1.37) was apparent. Ferulic acid inhibited basal and coupled respiration during L-malate oxidation either at acidic or alkaline pH, reducing also the amounts of pyruvate or oxaloacetate produced. The results suggest that the site of ferulic acid action is situated at some step that precedes the respiratory chain. An interference with the L-malate entry into the mitochondria could be an explanation for the effects of ferulic acid, but the possibility of a direct inhibition of both enzymes involved in L-malate oxidation cannot be ruled out.

Keywords: allelopathy; Glycine max; phenolic acid; respiration
Subjects: allelopathy, ferulic acid; ferulic acid, L-malate oxidation in mitochondria; Glycine max; malate oxidation in mitochondria; phenolic acid, respiration; respiration, ferulic acid; soybean, isolated mitochondria

Published: November 1, 1997  Show citation

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Sert, M.A., Ferraresi, M.L.L., Bernadelli, Y.R., Kelmer-Bracht, A.M., Bracht, A., & Ishii-Iwamoto, E.L. (1997). Effects of ferulic acid on L-malate oxidation in isolated soybean mitochondria. Biologia plantarum, 39(3), 345-350. doi: 10.1023/A:1001005711955
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