biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:523-530, 1997 | DOI: 10.1023/A:1001796902286

Somatic embryogenesis in Abies alba × Abies alba and Abies alba × Abies nordmanniana hybrids

B. Vooková1, A. Gajdošová1, R. Matúšová1
1 Institute of Plant Genetics, Slovak Academy of Sciences, Nitra, Slovak Republic

Maturation and germination of somatic embryos of hybrids A. alba × A. alba and A. alba × A. nordmanniana were followed by protein analysis of single embryogenic -suspensor masses (ESM) and analysis of storage protein accumulation during somatic embryo development. Very important step was one week pre-cultivation of ESM on medium with polyethylene glycol (PEG) and abscisic acid (ABA). Low osmotic potential of maturation medium and addition of ABA supported development of somatic embryo. Also partial drying of somatic embryo during following three weeks was needed for its normal development. In spite of morphologically fully developed, the somatic embryos were not physiologically ready for germination at least in terms of storage protein accumulation.

Keywords: fir; somatic embryo maturation; protein analysis
Subjects: Abies alba; Abies nordmanniana; fir, somatic embryogenesis; osmotic potential, somatic embryo maturation; proteins storage, somatic embryo; somatic embryogenesis, fir hybrids

Published: December 1, 1997  Show citation

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Vooková, B., Gajdošová, A., & Matúšová, R. (1997). Somatic embryogenesis in Abies alba × Abies alba and Abies alba × Abies nordmanniana hybrids. Biologia plantarum, 39(4), 523-530. doi: 10.1023/A:1001796902286
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