biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:297-299, 2000 | DOI: 10.1023/A:1002729032527

The Effect of Activated Charcoal Supplemented Media to Browning of In Vitro Cultures of Piper species

K. Madhusudhanan1, B.A. Rahiman2
1 Department of Bio-Sciences, Mangalore University, Mangalagangothri -, India
2 Department of Postgraduate Studies & Research in Life Sciences, Kuvempu University, Shimoga -, India

With the aim to effectively minimise the browning of tissue cultures of different Piper species (P. longum, P. attenuatum, P. betle, P. nigrum) explants from stem (nodal and internodal segments), petiole, and leaf were planted on Murashige and Skoog's basal medium supplemented with activated charcoal (AC). AC in the concentration of 200 mg dm-3 proved to be the best. Among the taxa studied, P. longum showed the least browning whereas, wild P. nigrum showed maximum browning.

Keywords: phenolics; plant regeneration
Subjects: in vitro culture, activated charcoal, browning; in vitro culture, phenolics, browning; pepper, in vitro regeneration, phenolics, browning; phenolics, in vitro regeneration, browning; Piper attenuatum; Piper betle; Piper longum; Piper nigrum

Published: August 1, 2000  Show citation

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Madhusudhanan, K., & Rahiman, B.A. (2000). The Effect of Activated Charcoal Supplemented Media to Browning of In Vitro Cultures of Piper species. Biologia plantarum, 43(2), 297-299. doi: 10.1023/A:1002729032527
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    References

    1. Bharadwaj, L., Ramawat, K.G.: Effect of antioxidants and adsorbents on tissue browning associated metabolism in Cocculus pendulus callus cultures.-Indian J. exp. Biol. 31: 715-718, 1993.
    2. Constantin, M.J., Henke, R.R., Mansur, M.A.: Effect of activated charcoal on callus growth and shoot organogenesis in tobacco.-In vitro 13: 293-296, 1977. Go to original source...
    3. Druart, P., De Wulf, O.: Activated charcoal catalyses sucrose hydrolysis during autoclaving.-Plant Cell Tissue Organ Cult. 32: 97-99, 1993. Go to original source...
    4. Ebert, A., Taylor, H.F.: Assessment of the changes of 2,4-dichlorophenoxyacetic acid concentrations in plant tissue culture media in presence of activated charcoal.-Plant Cell Tissue Organ Cult. 20: 165-172, 1990. Go to original source...
    5. Fridborg, G., Pedersen, M., Landstorm, L.E., Eriksson, T.: The effect of activated charcoal on tissue culture: absorption of metabolites inhibiting morphogenesis.-Physiol. Plant. 43: 104-106, 1978. Go to original source...
    6. George, E.F., Sherrington, P.D.: Plant Propagation by Tissue Culture. Exegetics Ltd., Eversley 1984.
    7. Jacquiot, C.: Application de la techniques de culture des tissus végétaux au l'étude de quelques problèmes de la physiologie de l'arbre.-Ann. Sci. forest. 21: 310-312, 1964. Go to original source...
    8. Krikorian, A.D.: Plant tissue culture-perceptions and realities.-Proc. Indian Acad. Sci. Plant Sci. 98: 425-464, 1988. Go to original source...
    9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    10. Nitsch, J.P., Strain, G.: Effet de diverses cytokinines sur le brunissement d'explantats de canne à sucre.-Compt. rend. Acad. Sci. Sér. D 269: 806-809, 1969.
    11. Scalbert, A., Monties, B., Favre, J.M.: Polyphenols of Quercus robur adult tree and in vitro grown calli and shoots.-Phytochemistry 27: 3483-3488, 1990. Go to original source...
    12. Weatherhead, M.A., Burdon, J., Henshaw, G.G.: Effect of activated charcoal as an additive to plant tissue culture media. Part 2.-Z. Pflanzenphysiol. 94: 399-406, 1979. Go to original source...

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