biologia plantarum

Biologia plantarum 45:609-611, 2002 | DOI: 10.1023/A:1022380827034

Callus Growth and Proline Accumulation in Response to Sorbitol and Sucrose-Induced Osmotic Stress in Rice

J.M. Al-Khayri¹, A.M. Al-Bahrany²

¹ Date Palm Research Center, College of Agriculture and Food Sciences, King Faisal University, Al-Hassa, Saudi Arabia

² Department of Horticulture, College of Agriculture and Food Sciences, King Faisal University, Al-Hassa, Saudi Arabia

This study investigated the influence of osmotic stress, induced by sorbitol and sucrose combinations, on growth and proline accumulation in callus cultures of rice (Oryza sativa L.). Dehusked mature seeds, cv. Hassawi, were induced to callus on MS medium supplemented with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 µM 6-furfurylaminopurine (kinetin). The medium also contained 29.2, 58.4, 87.6, and 116.8 mM sucrose combined with 0, 54.9, 109.8, and 164.7 mM sorbitol. Callus formation was observed in about 35 % of the cultured seeds irrespective of the sugar treatment. An increase in callus mass was observed as sucrose concentration increased reaching a maximum growth at 87.6 mM. Callus growth was enhanced in response to 54.9 mM sorbitol but at higher concentration it was inhibitory. Best callus growth was obtained on a medium containing 54.9 mM sorbitol combined with 87.6 mM sucrose. Increasing osmotic stress, as a consequence of increasing sucrose and sorbitol concentrations, induced proline accumulation and the highest concentration of proline, 5.8 µmol g^-1(f.m.), was obtained on 164.7 mM sorbitol combined with 116.8 mM sucrose.

Keywords: carbon source; Oryza sativa; sugar; tissue culture

Subjects: cytokinins; in vitro culture, bud and callus induction and growth; Oryza sativa; rice, callus growth, osmotic stress

Published: December 1, 2002  Show citation