biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:671-680, 2019 | DOI: 10.32615/bp.2019.073

The methylation pattern of DNA and complex correlations with gene expressions during TuMV infection in Chinese cabbage

J. YU1, L.-W. GAO1, Y. YANG1, C. LIU1, R.-J. ZHANG1, F.-F. SUN2, L.-X. SONG3, D. XIAO1, T.-K.LIU1, X.-L. HOU1, C.-W. ZHANG1,*
1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, P.R. China
2 Nanjing Vegetable Science Research Institute, Nanjing, 210095, P.R. China
3 Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, 210014, P.R. China

Chinese cabbage (Brassica rapa L. ssp. pekinensis) is one of the most important economic crops. However, its yield and quality can be severely threatened by Turnip mosaic virus (TuMV). Emerging evidence indicates that epigenetic mechanisms, especially DNA methylation, play an important role in regulating gene expression. Therefore, identification of resistance genes modified by DNA methylation during the virus infection would provide a critical clue for improving disease resistance breeding programs. Here, we present detailed analysis for the correlation of DNA methylation and gene expression involved in several anti-pathogen pathways. We also found that different methylation patterns exist in different methylation sites (CG, CHG, and CHH, where H represents A, G, or T) and genomic regions. Furthermore, we identified disease-resistant genes related to the nucleotide binding site-leucine-rich repeats family, auxin, salicylic acid signaling transduction, cell wall biosynthesis, and protein degradation among the different methylated genes (DMGs) suggesting that these genes may be modified by DNA methylation and work together to activate an immune response. The identified DMGs are a valuable resource for discovering resistance genes. Our study not only provides valuable data for future biotechnology research and epigenetic studies, but also helps to explore how the epigenetic mechanisms modify antiviral pathways.

Keywords: auxin, Brassica rapa, disease resistance, epigenetic mechanisms, NBS-LRR, salicylic acid.

Received: October 15, 2018; Revised: April 12, 2019; Accepted: May 9, 2019; Published online: November 8, 2019  Show citation

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YU, J., GAO, L.-W., YANG, Y., LIU, C., ZHANG, R.-J., SUN, F.-F., ... ZHANG, C.-W. (2019). The methylation pattern of DNA and complex correlations with gene expressions during TuMV infection in Chinese cabbage. Biologia plantarum, 63, Article 671-680. https://doi.org/10.32615/bp.2019.073
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