biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:776-784, 2019 | DOI: 10.32615/bp.2019.082

An overexpression of the AP2/ERF transcription factor from Iris typhifolia in Arabidopsis thaliana confers tolerance to salt stress

J. WU, J. ZHANG, X. LI, J. LIU, Z. NIU, L. WANG*
Northeast Forestry University, Harbin 150040, P.R. China

The roles of ethylene responsive factors (ERFs) and their positive and negative regulations of abiotic stress tolerance have been widely reported. This study reports the characterization of ItERF from Iris typhifolia Kitag with respect to molecular and functional properties. The 867 bp cDNA fragment of ItERF was cloned by reverse transcription PCR from I. typhifolia. Real-time quantitative PCR revealed that ItERF expression was induced in the roots, stems, and leaves of I. typhifolia after NaCl treatment, and that ItERF expressions were significantly higher in the leaves and roots than in the stems. A green fluorescent protein marker revealed that ItERF was located to the nucleus. Plant survival and root growth of ItERF transgenic Arabidopsis thaliana L. seedlings were much better than those of the wild type under NaCl stress. Malondialdehyde content in the transgenic lines was significantly lower than that in the wild type. Growth of yeast transformants showed an enhanced tolerance to salt stress than non-transformed yeast cells. All of the results verified that the expression of ItERF had effects on plant growth under salt stress.

Keywords: ethylene responsive factor, NaCl, transgenic plants.

Received: May 29, 2018; Revised: April 4, 2019; Accepted: May 3, 2019; Published online: December 10, 2019  Show citation

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WU, J., ZHANG, J., LI, X., LIU, J., NIU, Z., & WANG, L. (2019). An overexpression of the AP2/ERF transcription factor from Iris typhifolia in Arabidopsis thaliana confers tolerance to salt stress. Biologia plantarum, 63, Article 776-784. https://doi.org/10.32615/bp.2019.082
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    References

    1. Allen, M.D., Yamasaki, K., Ohme-Takagi, M., Tateno, M., Suzuki, M.: A novel mode of DNA recognition by a β-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA -. EMBO J. 17: 5485-5496, 1998. Go to original source...
    2. Ardie, S.W., Xie, L.N., Takahashi, R., Liu, S.K., Takano, T.: Cloning of a high-affinity K+ transporter gene PutHKT2;1 from Puccinellia tenuiflora and its functional comparison with OsHKT2;1 from rice in yeast and Arabidopsis. - J. exp. Bot. 60: 3491-3502, 2009. Go to original source...
    3. Bechtold, N., Ellis, J., Pelletier, G.: In planta Agrobacterium mediated gene transfer by infifiltration of adult Arabidopsis thaliana plants. Comptes Rendus Acad. Sci. Ser.III Life Sci. 316: 1194-1199, 1993.
    4. Bechtold, N., Pelletier, G.: In planta Agrobacterium mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Arabidopsis Protocols 82: 259-266, 1998. Go to original source...
    5. Chen, S.B., Songkumarn, P., Liu, J.L., Wang, G.L.: A versatile zero background T-vector system for gene cloning and functional genomics -. Plant Physiol. 150: 1111-1112, 2009. Go to original source...
    6. Fischer, U., Dröge-Laser, W.: Overexpression of NtERF5, a new member of the tobacco ethylene response transcription factor family enhances resistance to tobacco mosaic virus. - Mol. Plant Microbe Interact. 17: 1162-1171, 2004. Go to original source...
    7. Fujimoto, S.Y., Ohta, M., Usui, A., Shinshi, H., Ohme-Takagi, M.: Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. - Plant Cell 12: 393-404, 2000. Go to original source...
    8. Fukao, T., Yeung, E., Bailey-Serres, J.: The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice. - Plant Cell 23: 412-427, 2011. Go to original source...
    9. Gao, S., Zhang, H., Tian, Y.: Expression of TERF1 in rice regulates expression of stress-responsive genes and enhances tolerance to drought and high-salinity. - Plant Cell Rep. 27: 1787-1795, 2008. Go to original source...
    10. Gietz, R.D., Sugino, A.: New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking sis-base pair restriction sites. - Gene 74: 527-534, 1988. Go to original source...
    11. Gu, C.S., Liu, L.Q., Xu, C., Zhao, Y.H., Zhu, X.D., Huang, S.Z.: Reference gene selection for quantitative real-time RT-PCR normalization in Iris lactea var. chinensis roots under cadmium, lead, and salt stress conditions. - Sci. World J. 2014: 1-7, 2014. Go to original source...
    12. Igarashi, D., Ishida, S., Fukazawa, J., Takahashi, Y.: Proteins regulate intracellular localization of the bZIP transcriptional activator RSG. - Plant Cell 13: 2483-2497, 2001. Go to original source...
    13. Jung, J., Won, S.Y., Suh, S.C., Kim, H., Wing, R., Jenong, Y., Hwang, I., Kim, M.: The barley ERF-type transcription factor HvRAF confers enhanced pathogen resistance and salt tolerance in Arabidopsis. - Planta 225: 575-588, 2007. Go to original source...
    14. Lee, H.E., Shin, D.J., Park, S.R., Han, S.E., Jeong, M.J., Kwon, T.R., Lee, S.K., Park, S.C., Yi, B.Y., Kwon, H.B., Byun, M.O.: Ethylene responsive element binding protein 1 (StEREBP1) from Solanum tuberosum increases tolerance to abiotic stress in transgenic potato plants. - Biochem. biophys. Res. Commun. 353: 863-868, 2007. Go to original source...
    15. Mishra, S., Phukan, U.J., Tripathi, V., Singh, D.K., Luqman, S., Shukla, R.K.: PsAP2 an AP2/ERF family transcription factor from Papaver somniferum enhances abiotic and biotic stress tolerance in transgenic tobacco. - Plant mol. Biol. 89: 173-186, 2015. Go to original source...
    16. Mizoia, J., Shinozaki, K., Yamaguchi-Shinozaki, K.: AP2/ERF family transcription factors in plant abiotic stress responses. - Biochim. Biophys. Acta 1819: 86-96, 2012. Go to original source...
    17. Nakano, T., Suzuki, K., Fujimura, T., Shinshi, H.: Genome-wide analysis of the ERF gene family in Arabidopsis and rice. - Plant Physiol. 140: 411-432, 2006. Go to original source...
    18. Núñez-Pastrana, R., Anderson, J., Singh, K.B.: Ethylene response factors and their role in plant defense. - CAB Rev. 8008: 1-12, 2013. Go to original source...
    19. Ohta, M., Matsui, K., Hiratsu, K., Shinshi, H., Ohme-Takagi, M.: Repression domains of class II ERF transcriptional repressors share an essential motif for active repression. - Plant Cell 13: 1959-1968, 2001. Go to original source...
    20. Okamuro, J.K., Caster, B., Villarroel, R., Van Montagu, M., Jofuku, K.D.: The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc. nat. Acad. Sci. USA 94: 7076-7081, 1997. Go to original source...
    21. Oñate-Sánchez, L., Singh, K.B.: Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection. - Plant Physiol. 128: 1313-1322, 2002. Go to original source...
    22. Park, H.Y., Seok, H.Y., Woo, D.H., Lee, S.Y.: AtERF71/HRE2 transcription factor mediates osmotic stress response as well as hypoxia response in Arabidopsis. - Biochem. biophys. Res. Commun. 414: 135-141, 2011. Go to original source...
    23. Park, J.M., Park, C.J., Lee, S.B., Ham, B.K., Shin, R., Paek, K.H.: Overexpression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco. - Plant Cell 13: 1035-1046, 2001. Go to original source...
    24. Pfaffl, M.W.: A new mathematical model for relative quantification in real-time RT-PCR. Nucl. Acid. Res. 29: e45, 2001. Go to original source...
    25. Sambrook, J., Fritsch, E., Maniatis, T.: Molecular Cloning: A laboratory Manual. 2nd Ed. - Cold Spring Habor Press, Cold Spring Harbor - New York 1989.
    26. Sharma, M.K., Kumar, R., Solanke, A.U., Sharma, R., Tyagi, A.K., Sharma, A.K.: Identification, phylogeny, and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato. - Mol. Genet. Genomics 284: 455-475, 2010. Go to original source...
    27. Song, C.P., Agarwal, M., Ohta, M., Guo, Y., Halfter, U., Wang, P., Zhu, J.K.: Role of an Arabidopsis AP2/EREBP-type transcriptional repressor in abscisic acid and drought stress responses. - Plant Cell 17: 2384-2396, 2005. Go to original source...
    28. Tournier, B., Sanchez-Ballesta, M.T., Jones, B., Pesquet, E., Regad, F., Latché, A., Pech, J.-C., Bouzayen, M.: New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element. - FEBS Lett. 550: 149-154, 2003. Go to original source...
    29. Wang, X., Hou, C., Zheng, K., Li, Q., Chen, S., Wang, S.: Overexpression of ERF96, a small ethylene response factor gene enhances salt tolerance in Arabidopsis. - Biol. Plant. 61: 693-701, 2017. Go to original source...
    30. Wang, Y.C., Gao, C.Q., Liang, Y,N., Wang, C., Yang, C.P., Liu, G.F.: A novel bZIP gene from Tamarix hispida mediates physiological responses to salt stress in tobacco plants. J Plant Physiol. 167: 222-230, 2010. Go to original source...
    31. Wu, F.H., Shen, S.C., Lee, L.Y., Lee, S.H., Chan, M.T., Lin, C.S.: Tape-Arabidopsis sandwich - a simpler Arabidopsis protoplast isolation method. - Plant Methods 5: 1-10, 2009. Go to original source...
    32. Yang, Z., Tian, L.N., Latoszek-Green, M., Brown, D., Wu, K.Q.: Arabidopsis ERF4 is a transcriptional repressor capable of modulating ethylene and abscisic acid responses. - Plant mol. Biol. 58: 585-596, 2005. Go to original source...
    33. Zhai, Y., Wang, Y., Li, Y.J., Lei, T.T., Yan, F., Su, L.T., Li, X.W., Zhao, Y., Sun, X., Li, J.W., Wang, Q.Y.: Isolation and molecular characterization of GmERF7, a soybean ethylene-response gene. - Gene 513: 174-183, 2013. Go to original source...
    34. Zhang, G.Y., Chen, M., Chen, X.P., Xu, Z.S., Li, L.C., Guo, J.M., Ma, Y.Z.: Isolation and characterization of a novel EAR-motif containing gene GmERF4 from soybean (Glycine max L.). - Mol. Biol. Rep. 37: 809-818, 2010. Go to original source...
    35. Zhang, G.Y., Chen, M., Li, L.C., Chen, X.P., Guo, J.M., Ma, Y.Z.: Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco. - J. exp. Bot. 60: 3781-3796, 2009. Go to original source...
    36. Zhou, J., Tang, X., Martin, G.B.: The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. - EMBO J. 16: 3207-3218, 1997. Go to original source...

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