biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 1:93, 1959 | DOI: 10.1007/BF02927043

The Cytomorphological Effects of Low Temperatures Studied by Temperated Fixatives

Karel Beneš1
1 Department of Plant Physiology, Institute of Biology, Czechoslovak Academy of Sciences, Praha

The mechanism of the cytomorphological effects of low temperatures was studied by the fixation of young leaves ofTriticum vulgare with temperated alcoholic fixatives. Some specimens were plunged into liquid nitrogen, allowed to thaw and fixed; other objects were plunged into liquid nitrogen and fixed without thawing; control objects were fixed directly. At various temperatures of fixatives (-76, -27,+27,+58° C) different results were observed on the objects pretreated in the same manner. It was thus demonstrated that the destruction processes occur, in this case, during thawing.

Received: July 18, 1958; Published: June 1, 1959  Show citation

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Beneš, K. (1959). The Cytomorphological Effects of Low Temperatures Studied by Temperated Fixatives. Biologia plantarum, 1(2), 93. doi: 10.1007/BF02927043
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