Biologia plantarum, 2026 (vol. 70)
Original Papers
Optimized protocol for in vitro indirect organogenesis and shoot regeneration of Platycladus orientalis
Luis LUCENA-SORIANO, Ana María FERNÁNDEZ-OCAÑA
Biologia plantarum 70:1-10, 2026 | DOI: 10.32615/bp.2026.001 
Background: Platycladus orientalis L. is a drought-tolerant conifer valued for its ornamental and medicinal properties. However, efficient regeneration systems for this species remain limited, hindering its propagation and conservation. Aims: The aim of this study was to develop a reliable protocol for indirect organogenesis of Platycladus orientalis under in vitro conditions, evaluating the influence of explant type, culture medium, light exposure, and pretreatment on regeneration efficiency. Methods: Cotyledon, hypocotyl, and radicle explants were cultured on different media formulations. The effects of light and darkness during...
RT-qPCR analysis of genotype-specific beet yellows virus accumulation in sugar beet
Emad IBRAHIM, Dilara BOZDOĞAN, Jiban KUMAR KUNDU
Biologia plantarum 70:11-17, 2026 | DOI: 10.32615/bp.2026.002
Background: Rapid and objective characterisation of viral accumulation requires methods combining fast detection and temporal resolution. Aims: Develop a two-phase approach for screening sugar beet genotypes for beet yellows virus (BYV) accumulation dynamics. Methods: Phase 1 employed one-enzyme RTX-PCR for rapid BYV detection, followed by single-time-point RT-qPCR at 30 days post-inoculation (dpi) to screen ten genotypes. Phase 2 performed time-course RT-qPCR at six time points (10-60 dpi) on two contrasting genotypes selected from Phase 1, with assay performance validated by a standard curve. Results: One-enzyme RTX-PCR confirmed BYV infection, enabling...
Composition of organic acids secreted by alpine shrub roots and its influencing factors
Chen-Kang XI, Ling ZHU, Naveed AHMED, Yong-Heng GAO
Biologia plantarum 70:18-26, 2026 | DOI: 10.32615/bp.2026.003
Background and aims: There appears to be a strong correlation between the rapid proliferation of shrubs in the alpine grassland of the Qinghai-Tibet Plateau and the root exudates of these plants. The dynamics of root exudates during shrub development, however, have been the subject of few investigations. Methods: This work examined Lonicera thibetica, a plant found on the eastern edge of the Qinghai-Tibet Plateau. It focused on the root systems of 5-, 10-, 15-, and 20-year-old L. thibetica plants and how their primary organic acid components changed with the seasons and other circumstances. Results: The findings revealed that the primary...
Integrated molecular and serological diagnostics for surveillance of the quarantine virus tomato brown rugose fruit virus
Hana HOFFMEISTEROVÁ, Emad IBRAHIM, Jakub DUŠEK, Qinhai LIU, Min ZHU, Xiaorong TAO, Noemi ČEŘOVSKÁ, Tomáš MORAVEC, Jiban Kumar KUNDU
Biologia plantarum 70:27-37, 2026 | DOI: 10.32615/bp.2026.004
Background: The tomato brown rugose fruit virus (Tobamovirus fructirugosum, ToBRFV) is an emerging tobamovirus that has quickly become a significant obstacle to the production of tomatoes and peppers worldwide. It is now classified as a regulated quarantine pathogen. Effective containment requires rapid, reliable, inexpensive, and safe diagnostic protocols for routine screening in laboratories and production systems. Aims: We aimed to thoroughly evaluate integrated molecular and serological diagnostic methods for ToBRFV and develop biosafe positive controls suitable for high-throughput and decentralized applications. Methods: We evaluated the...