Biologia plantarum, 2025 (vol. 69)
Reviews
Structural and functional insights into NAC transcription factors in tomato stress responses and development
Shan FU, Ying LI, Fei LIU, Chunyan LU, Tiantian LIU, Songzhou TIAN, Xiaolan ZHANG, Pingan GUO, Liang CHEN
Biologia plantarum 69:49-57, 2025 | DOI: 10.32615/bp.2025.004 
In natural environments, the growth and development of plants are frequently impeded by a variety of stresses. These can be categorized into biotic stresses, such as those caused by fungi and bacteria, and abiotic stresses, including factors like low temperature, drought, and salinity (Zhu, 2016). These stresses impact plant photosynthesis, osmotic adjustment, and nutrient uptake, thereby inhibiting plant growth and ultimately resulting in a reduced crop yield and quality. To adapt to the dynamic changes in the environment, plants have evolved a series of complex defense mechanisms that are precisely regulated at the molecular, cellular, biochemical,...
Original Papers
Effects of NO3-/NH4+ ratios on growth, enzyme activity and nitrogen assimilation-related gene expression in Toona sinensis seedlings
Xiaopu SHI, Taotao SHAO, Beibei MA, Juan WANG, Mingqin FAN, Hu ZHAO
Biologia plantarum 69:12-20, 2025 | DOI: 10.32615/bp.2025.002
Nitrogen is an essential nutrient for plants. Different nitrate (NO3-)/ammonium (NH4+) ratios have different effects on plant growth. However, the underlying mechanism in Toona sinensis remains unclear. Thus, we determined the effects of five different NO3-/NH4+ ratios (16/0, 12/4, 8/8, 4/12, and 0/16, denoted T1, T2, T3, T4, and T5, respectively) in nutrient media on T. sinensis seedling growth. When the nitrogen source was NH4+ alone (T5) or NO3- alone (T1), the soluble protein content in the leaves...
Physiological, anatomical, and transcriptomic analyses reveal the effects of acid rain stress on Akebia trifoliata and the mitigation potential of exogenous curcumin
Xingmei TAO, Kai WANG, Xiaoxu BI, Yongfu ZHANG
Biologia plantarum 69:21-37, 2025 | DOI: 10.32615/bp.2025.003
This study investigated the impacts of acid rain stress on Akebia trifoliata and the mitigation effects of exogenous curcumin (CUR) using integrated physiological, anatomical, and transcriptomic analyses. Acid rain stress significantly decreased chlorophyll content (total chlorophyll by 64.8%), leaf epidermal thickness (upper and lower epidermis by 58.9 and 35.6%), and starch content (by 63.9%), while increasing oxidative stress markers (MDA by 82.6%; ROS production by 345.8%) and content of osmolytes (proline by 64.4%). A. trifoliata counteracted acid rain stress by enhancing superoxide dismutase (SOD) and catalase (CAT) activities,...
Sulfur dioxide promotes wheat seed germination under drought stress and regulates antioxidant metabolism in wheat
Nan-Nan LIU, Zi-Xu LU, Xi-Kai GUO, Gui-Lin ZHOU, Yi-Ran XUAN, Zhi-Yan WANG, Zhi-Kun YU, Gai-Fang YAO, Juan LI, Rong-Fang XU, Kang-Di HU, Hua ZHANG
Biologia plantarum 69:58-67, 2025 | DOI: 10.32615/bp.2025.006
With the drastic changes in global climate and the increasing frequency of extreme weather events, abiotic stress poses a significant threat to future food crop production and is a major contributor to crop yield reduction (Ahmad et al., 2010). Wheat, one of the world's primary food crops, is susceptible to both abiotic and biotic stresses at all stages of its growth and development. Numerous studies have indicated that drought stress severely limits the growth and productivity of wheat (Xue et al., 2014; Faran et al., 2019). Therefore, understanding the effects of drought stress on wheat grain germination and developing strategies to mitigate the...
Special Issue of “Czech Society of Experimental Plant Biology”
Moderate drought stress increases resistance of Brassica napus to subsequent infection by Leptosphaeria maculans
Barbora JINDŘICHOVÁ, Marzieh MOHRI, Tetiana KALACHOVA, Romana POSPÍCHALOVÁ, Pavel RYŠÁNEK, Lenka BURKETOVÁ
Biologia plantarum 69:1-11, 2025 | DOI: 10.32615/bp.2025.001
Plants have developed adaptive strategies to cope with environmental stresses, but mechanisms effective under one stress may be counterproductive under others. This study investigates the effect of moderate drought stress pretreatment on the resistance of Brassica napus to Leptosphaeria maculans, the pathogen causing blackleg disease. B. napus plants were exposed to varying durations of drought stress, followed by a 24-h recovery period before inoculation with L. maculans. The results demonstrate a priming effect of the drought pretreatment, with a reduction in necrotic lesions in cotyledons compared to non-stressed controls....
GoldenBraid-compatible infectious clone of apple latent spherical virus (ALSV) and its use for virus-induced gene silencing
Hana HOFFMEISTEROVÁ, Jakub DUŠEK, Emad IBRAHIM, Tomáš MORAVEC, Jiban Kumar KUNDU
Biologia plantarum 69:38-48, 2025 | DOI: 10.32615/bp.2025.005
Virus-induced gene silencing (VIGS) is a technological process in which the expression of a plant target gene is down-regulated by inoculating a plant with a recombinant virus-based vector carrying part of the coding sequence of the target gene (Baulcombe, 1999a; Burch-Smith et al., 2004). VIGS uses an RNA silencing-based defence mechanism in which double-stranded RNAs (dsRNAs) of viral origin, as templates, are processed into small interfering RNAs by Dicer-like enzymes. The resulting siRNA is incorporated into an RNA-induced silencing complex, which leads to the degradation of the RNA (viral RNA, mRNA) with sequences complementary to the siRNA. Thus,...