biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 5:298, 1963 | DOI: 10.1007/BF02930910

Polyphenol oxidase in the roots ofCucurbita pepo L.

Miroslav Dvořák1, Jana Černohorská1
1 Department of Plant Physiology and Soil Biology of the Faculty of Natural Science, Charles University, Praha

From the roots ofCucurbita pepo L. an acetone preparation was obtained with a polyphenol-oxidase activity towards pyrogallol, less marked towards pyrocatechin and DOPA. It is competitively inhibited by DIECA and KCN, but is not indibited by NaN3, which is evidence that it is a copper enzyme of the polyphenol oxidase type. No quantitative differences between the two variants "NS" and "-Ca" were found nor qualitative differences expressed by the affinity for the substrate.

Received: September 19, 1962; Published: December 1, 1963  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Dvořák, M., & Černohorská, J. (1963). Polyphenol oxidase in the roots ofCucurbita pepo L. Biologia plantarum, 5(4), 298. doi: 10.1007/BF02930910
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bladegroen, W.: Einführung in die Energetik und Kinetik biologischer Vorgänge. Basel, 1955
    2. Dvořák, M.: The effect of mineral nutrition on the respiratory systems ofCucurbita roots.- Acta Univ. Carol.-Biologica, No. 2, 155-183, 1960.
    3. Dvořák, M.: Participation of ascorbate oxidase in the respiration of theCucurbita roots.-Biol. Plant.5: 287-297, 1963. Go to original source...
    4. James, W. O.: The use of respiratory inhibitors.-Ann. Rev. Plant Physiol.4: 59-90, 1953a. Go to original source...
    5. James, W. O.: The terminal oxidases of plant respiration.-Biol. Rev.28: 245-260, 1953b. Go to original source...
    6. James, W. O., Roberts, E. A. H., Beevers, H., De Kock, P. C.: The secondary oxidation of aminoacids by the catecholoxidase of Belladona.-Biochem. J.43: 623-636, 1948. Go to original source...
    7. Keilin, D.: The action of sodium azide on cellular respiration and on some catalytic oxidation reactions.-Proc. R. Soc. (London) B121: 165-173, 1936. Go to original source...
    8. Warner, C.: A competitive inhibitor of tyrosinase.-Austr. J. Sci. Res. B4: 554-560, 1951. Go to original source...

    Return to the content