biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 5:310-317, 1963 | DOI: 10.1007/BF02930912

Quantitative determination of cyanogenesis in plants

Václav Pokorný1,2
1 Institute of Genetics, Faculty of Natural Sciences, Charles University, Praha
2 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

A method suitable for serial determinations in genetic experiments is described. Colorimetric determination of HCN (by 1-phenyl-3-methyl-5-pyra-zolone and pyridine, phenolphthalin or sodium picrate) facilitates processing of samples releasing 0.2 μg to 20 μg of HCN. Before hydrolysis of the glycoside the oil content in the seeds can be determined. Variance analysis of the cyanogenic properties of the particular seeds of two sorts of flax with an average HCN content of 0.4 and 0.9 μg shows that the differences between plants and between capsulae of the same plant can be determined.

Received: October 5, 1962; Published: December 1, 1963  Show citation

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Pokorný, V. (1963). Quantitative determination of cyanogenesis in plants. Biologia plantarum, 5(4), 310-317. doi: 10.1007/BF02930912
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    References

    1. American Public Health Association: Standard Methods for the Examination of Water Sewage and Industrial Wastes-10th Ed. 293-302, 1955 (New York).
    2. Bourquelot, E., Danjou, E.: Sur la sambunigrine, glucoside cyanhydrique nouveau retiré des feuilles de Surreau noir.-C. R. Acad. Sci., Paris,141: 598-600, 1905.
    3. Coop, J. E.: Cyanogenesis in white clover (Trifolium repens). III. A study of linamarase, the enzym which hydrolyses lotaustralin.-N. Z. Jour. Sci. & Tech.,22: 71B-83B, 1940.
    4. Corkill, L.: Cyanogenesis in white clover (Trifolium repens) I. Cyanogenesis in single plants.- N. Z. Jour. Sci & Tech.,22: 65B-67B, 1940.
    5. Epstein, J.: Estimation of microquantities of cyanide.-Anal. Chem.,19: 277-274, 1947. Go to original source...
    6. Guignard, M. L.: Le haricot à acide cyanhydrique,Phaseolus lunatus L.-C. R. Acad. Sci., Paris,142: 545-553, 1906.
    7. Krüger, F.: Ein neuer Mikro-Respirationsapparat zur laufenden manometrischen Bestimmung des respiratorischen Quotienten.-Z. vergl. Physiol.,21: 249-266, 1935. Go to original source...
    8. Lüedtke, M.: Über das Linamarin des Leinsamens und seine Bestimmung.-Biochem., Z.,322: 310-319, 1952. Biochem. Z. Über das Linamarin des Flachsstengels.-Biochem. Z.,323: 428/436, 1953.
    9. Lüedtke, M., Hahn, H.: Über den Linamaringehalt gesunder und von Colletotrichum lini befallener junger Leinpflanzen.-Biochem. Z.,324: 433-442, 1953.
    10. Mirande, M.: Influence excercéc par certaines vapeurs sur la cyanogenèse végétale.-C. R. Acad. Sci., Paris,149: 140-142, 1909.
    11. Robbie, W. A.: The quantitative control of cyanide in manometric experimentation.-J. Cell. comp. Physiol.,27: 181-209, 1946. Go to original source...
    12. Rosenthaler, L.: Blausäureglucoside (Oxynitriglucoside). InKlein, G.: Handbuch der Pflanzenanalyse, Bd.3: 1036-1057, 1957 (Wien).
    13. Seifert, P.: Blausäureverbindungen. InPeach K., Tracey M. V.: Moderne Methoden der Pflanzenanalyse, Bd.4: 676-638, 1955 (Berlin). Go to original source...

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